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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PPA2 Monoclonal antibody

PPA2 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1C2E3

Cat no : 68468-1-Ig

Synonyms

FLJ20459, HSPC124, PPA2, PPase 2, pyrophosphatase (inorganic) 2, Pyrophosphatase SID6 306, SID6 306

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PPA2 Monoclonal antibody (68468-1-Ig)

    WB analysis using 68468-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68468-1-Ig (PPA2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • Western Blot (WB) analysis of 4T1 cells using PPA2 Monoclonal antibody (68468-1-Ig)

    WB analysis of 4T1 using 68468-1-Ig

    4T1 cells were subjected to SDS PAGE followed by western blot with 68468-1-Ig (PPA2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inU2OS cells, 4T1 cells, LNCaP cells, HeLa cells, HEK-293T cells, Jurkat cells, Raji cells, K-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68468-1-Ig targets PPA2 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PPA2 fusion protein Ag10125 相同性解析による交差性が予測される生物種
Full Name pyrophosphatase (inorganic) 2
Calculated molecular weight305aa,35 kDa; 334aa,38 kDa
Observed molecular weight34 kDa
GenBank accession numberBC057219
Gene symbol PPA2
Gene ID (NCBI) 27068
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Pyrophosphatase 2 (PPA2) is a mitochondrial-located pyrophosphatase (PPase) that hydrolyzes inorganic pyrophosphate (PPi), generated by numerous nucleotide-dependent reactions, into two orthophosphate molecules (PMID: 34400813). PPA2 is a cardiomyopathy-associated protein, which has a greater physiological importance in mitochondrial function (PMID: 27523597).

Protocols

Product Specific Protocols
WB protocol for PPA2 antibody 68468-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols