Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, mouse heart tissue, rat heart tissue, rat liver tissue |
| Positive IHC detected in | human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 4 publications below |
| WB | See 6 publications below |
| IHC | See 1 publications below |
| IF | See 2 publications below |
| IP | See 2 publications below |
Product Information
11006-1-AP targets PPAN in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, xenopus |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PPAN fusion protein Ag1419 相同性解析による交差性が予測される生物種 |
| Full Name | peter pan homolog (Drosophila) |
| Calculated molecular weight | 53 kDa |
| Observed molecular weight | 55-58 kDa |
| GenBank accession number | BC009833 |
| Gene Symbol | PPAN |
| Gene ID (NCBI) | 56342 |
| RRID | AB_2168040 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NQ55 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PPAN (or SSF, SSF1 or SSF2) gene is widely expressed, and encodes peter pan homolog protein which may be localized in nucleus. Two isoforms are produced through alternative splicing. So far the characterization of this protein is largely unknown, multiple phosphorylation sites has been revealed based on high-throughput phosphorylation analysis. This antibody is raised against the C-terminal of huaman PPAN and recognize bands around 55 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PPAN antibody 11006-1-AP | Download protocol |
| IHC protocol for PPAN antibody 11006-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
EMBO J The long non-coding RNA Paupar promotes KAP1-dependent chromatin changes and regulates olfactory bulb neurogenesis. | ||
Development Peter Pan functions independently of its role in ribosome biogenesis during early eye and craniofacial cartilage development in Xenopus laevis. | ||
Cancers (Basel) Nucleolar Stress Functions Upstream to Stimulate Expression of Autophagy Regulators.
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J Biol Chem The Wnt target Peter Pan defines a novel p53-independent nucleolar stress response pathway.
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Biol Cell Pescadillo homologue 1 and Peter Pan function during Xenopus laevis pronephros development. | ||
Cells Loss of Peter Pan (PPAN) Affects Mitochondrial Homeostasis and Autophagic Flux.
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