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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PPAP2B Polyclonal antibody (27391-1-AP)

    WB analysis using 27391-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27391-1-AP (PPAP2B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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PPAP2B Polyclonal antibody

PPAP2B Polyclonal Antibody for WB, ELISA
Cat No. 27391-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

PAP2 beta, PAP2 b, PAP 2b, LPP3, EC:3.1.3.-

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inU-87 MG cells, human placenta tissue, mouse cerebellum tissue, mouse lung tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27391-1-AP targets PPAP2B in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PPAP2B fusion protein Ag26422 相同性解析による交差性が予測される生物種
Full Name phosphatidic acid phosphatase type 2B
Calculated molecular weight 35 kDa
Observed molecular weight30-40 kDa
GenBank accession numberBC009196
Gene Symbol PPAP2B
Gene ID (NCBI) 8613
RRIDAB_3669598
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO14495
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Phosphatidic acid phosphatase type 2B (PPAP2B), also known as Lipid phosphate phosphatase 3 (LPP3), is a member of the phosphatidic acid phosphatase (PAP) family. PPAP2B is an integral membrane protein that inactivates lysophosphatidic acid, was implicated in coronary artery disease (CAD) by genomewide association studies (PMID: 26034042). PPAP2B can be detected at least two immunoreactive bands with different mobility, ranging from 36 to 50 kDa, which may reflect oligomer formation and/or post-translational protein modifications, such as glycosylation (PMID: 28364255).

Protocols

Product Specific Protocols
WB protocol for PPAP2B antibody 27391-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols