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Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using PPARD Monoclonal antibody (60193-1-Ig)

    WB analysis of A549 using 60193-1-Ig (same clone as 60193-1-PBS)

    WB result of PPARD antibody (60193-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PPARD transfected A549 cells. This data was developed using the same antibody clone with 60193-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PPARD Monoclonal antibody (60193-1-Ig)

    WB analysis using 60193-1-Ig (same clone as 60193-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60193-1-Ig (PPARD antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60193-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig adipose tissue using PPARD Monoclonal antibody (60193-1-Ig)

    WB analysis of pig adipose using 60193-1-Ig (same clone as 60193-1-PBS)

    pig adipose tissue were subjected to SDS PAGE followed by western blot with 60193-1-Ig (PPARD antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60193-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

PPARD Monoclonal antibody, PBS Only

PPARD Monoclonal Antibody for WB, Indirect ELISA
Cat No. 60193-1-PBS
Clone No.1B10E1

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig

Applications

WB, Indirect ELISA

Synonyms

PPAR delta, PPARB, PPAR beta, Peroxisome proliferator-activated receptor delta, Peroxisome proliferator-activated receptor beta

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60193-1-PBS targets PPARD in WB, Indirect ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen PPARD fusion protein Ag16770 相同性解析による交差性が予測される生物種
Full Name peroxisome proliferator-activated receptor delta
Calculated molecular weight 50 kDa
Observed molecular weight 54 kDa
GenBank accession numberBC002715
Gene Symbol PPARD
Gene ID (NCBI) 5467
RRIDAB_10896827
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ03181
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Peroxisome proliferator-activator receptor delta(PPARD), a ligand-activated transcription factor, belongs to the PPARs family, which get its name for their chemicals that induce proliferation of peroxisomes, organelles that contributes to the oxidation of fatty acids. PPARD is a receptor for peroxisome proliferator and once activated by a ligand, it binds to and activates the promoter elements of target genes, such as acylCoA oxidase gene. But it act as a repressor for the NPC1L1 gene.