Validation Data Gallery
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Tested Applications
| Positive WB detected in | HEK-293 cells, SH-SY5Y cells, rat liver tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 15 publications below |
| CoIP | See 1 publications below |
Product Information
22378-1-AP targets PPARGC1B in WB, CoIP, ELISA applications and shows reactivity with human, rat, mouse samples.
| Tested Reactivity | human, rat, mouse |
| Cited Reactivity | human, mouse, pig, goat, fish, megalobrama amblycephala |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PPARGC1B fusion protein Ag17566 相同性解析による交差性が予測される生物種 |
| Full Name | peroxisome proliferator-activated receptor gamma, coactivator 1 beta |
| Calculated molecular weight | 1023 aa, 113 kDa |
| Observed molecular weight | 100-110 kDa |
| GenBank accession number | BC132971 |
| Gene Symbol | PPARGC1B |
| Gene ID (NCBI) | 133522 |
| RRID | AB_2879093 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q86YN6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PPARGC1B antibody 22378-1-AP | Download protocol |
| IP protocol for PPARGC1B antibody 22378-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Redox Biol miR-484 mediates oxidative stress-induced ovarian dysfunction and promotes granulosa cell apoptosis via SESN2 downregulation | ||
Br J Pharmacol Andrographolide prevents bone loss via targeting ERRalpha-regulated metabolic adaption of osteoclastogenesis. | ||
EMBO Rep Mitophagy receptor FUNDC1 is regulated by PGC-1α/NRF1 to fine tune mitochondrial homeostasis.
| ||
J Anim Sci Biotechnol miR-25 modulates triacylglycerol and lipid accumulation in goat mammary epithelial cells by repressing PGC-1beta. | ||
Nutrients Novel Choline-Deficient and 0.1%-Methionine-Added High-Fat Diet Induces Burned-Out Metabolic-Dysfunction-Associated Steatohepatitis with Inflammation by Rapid Immune Cell Infiltration on Male Mice | ||
FASEB J Hypolipidemic effect of oleanolic acid is mediated by the miR-98-5p/PGC-1β axis in high-fat diet-induced hyperlipidemic mice.
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