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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PPBP,NAP2 Polyclonal antibody

PPBP,NAP2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13313-1-AP

Synonyms

B TG1, Beta TG, C X C motif chemokine 7, CTAP III, CTAP3, CTAPIII, CXCL7, LA PF4, LDGF, MDGF, NAP 2, NAP 2(1 63), NAP-2, PBP, Platelet basic protein, PPBP, SCYB7, Small inducible cytokine B7, TC1, TC2, TGB, TGB1, THBGB, THBGB1

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using PPBP,NAP2 Polyclonal antibody (13313-1-AP)

    WB analysis of mouse liver using 13313-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 13313-1-AP (PPBP,NAP2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human plasma using PPBP,NAP2 Polyclonal antibody (13313-1-AP)

    WB analysis of human plasma using 13313-1-AP

    human blood were subjected to SDS PAGE followed by western blot with 13313-1-AP (PPBP,NAP2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using PPBP,NAP2 Polyclonal antibody (13313-1-AP)

    WB analysis of human liver using 13313-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 13313-1-AP (PPBP,NAP2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using PPBP,NAP2 Polyclonal antibody (13313-1-AP)

    IHC staining of human placenta using 13313-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13313-1-AP (PPBP,NAP2 antibody at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using PPBP,NAP2 Polyclonal antibody (13313-1-AP)

    IHC staining of human placenta using 13313-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13313-1-AP (PPBP,NAP2 antibody at dilution of 1:50 (under 40x lens).

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Tested Applications

Positive WB detected inmouse liver tissue, human liver tissue, human plasma tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

13313-1-AP targets PPBP,NAP2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PPBP,NAP2 fusion protein Ag4147 相同性解析による交差性が予測される生物種
Full Name pro-platelet basic protein (chemokine (C-X-C motif) ligand 7)
Calculated molecular weight 128 aa, 14 kDa
Observed molecular weight 8-14 kDa
GenBank accession numberBC028217
Gene symbol PPBP
Gene ID (NCBI) 5473
RRIDAB_10597238
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PPBP, slso named as NAP2, or β-Thromboglobulin, is a platelet-derived growth factor that belongs to the CXC chemokine family. This growth factor is a potent chemoattractant and activator of neutrophils. It has been shown to stimulate various cellular processes including DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, prostaglandin E2 secretion, and synthesis of hyaluronic acid and sulfated glycosaminoglycan. It also stimulates the formation and secretion of plasminogen activator by synovial cells.

Protocols

Product Specific Protocols
WB protocol for PPBP,NAP2 antibody 13313-1-APDownload protocol
IHC protocol for PPBP,NAP2 antibody 13313-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nat Commun

Mutant Huntingtin stalls ribosomes and represses protein synthesis in a cellular model of Huntington disease.

Authors - Mehdi Eshraghi
View Article