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Species Reactivity

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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using PPCS Polyclonal antibody (18001-1-AP)

    WB analysis of HeLa using 18001-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 18001-1-AP (PPCS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using PPCS Polyclonal antibody (18001-1-AP)

    IHC staining of human kidney using 18001-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 18001-1-AP (PPCS antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using PPCS Polyclonal antibody (18001-1-AP)

    IHC staining of human kidney using 18001-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 18001-1-AP (PPCS antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using PPCS Polyclonal antibody (18001-1-AP)

    IHC staining of human placenta using 18001-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 18001-1-AP (PPCS antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using PPCS Polyclonal antibody (18001-1-AP)

    IHC staining of human placenta using 18001-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 18001-1-AP (PPCS antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using PPCS Polyclonal antibody (18001-1-AP)

    IHC staining of human testis using 18001-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 18001-1-AP (PPCS antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using PPCS Polyclonal antibody (18001-1-AP)

    IHC staining of human testis using 18001-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 18001-1-AP (PPCS antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using PPCS Polyclonal antibody (18001-1-AP)

    IHC staining of human spleen using 18001-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 18001-1-AP (PPCS antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using PPCS Polyclonal antibody (18001-1-AP)

    IHC staining of human spleen using 18001-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 18001-1-AP (PPCS antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using PPCS Polyclonal antibody (18001-1-AP)

    IHC staining of human lung using 18001-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 18001-1-AP (PPCS antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using PPCS Polyclonal antibody (18001-1-AP)

    IHC staining of human lung using 18001-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 18001-1-AP (PPCS antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using PPCS Polyclonal antibody (18001-1-AP)

    IHC staining of human ovary using 18001-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 18001-1-AP (PPCS antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using PPCS Polyclonal antibody (18001-1-AP)

    IHC staining of human ovary using 18001-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 18001-1-AP (PPCS antibody) at dilution of 1:100 (under 40x lens).

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PPCS Polyclonal antibody

PPCS Polyclonal Antibody for WB, IHC, ELISA
Cat No. 18001-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Synonyms

COAB, PPC synthetase, PPCS

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inhuman kidney tissue, human lung tissue, human ovary tissue, human placenta tissue, human spleen tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

18001-1-AP targets PPCS in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PPCS fusion protein Ag12491 相同性解析による交差性が予測される生物種
Full Name phosphopantothenoylcysteine synthetase
Calculated molecular weight 311 aa, 34 kDa
Observed molecular weight 34 kDa
GenBank accession numberBC106064
Gene Symbol PPCS
Gene ID (NCBI) 79717
RRIDAB_10548725
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9HAB8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PPCS(Phosphopantothenate--cysteine ligase) is also named as COAB and belongs to the PPC synthetase family. It catalyzes the first step in the biosynthesis of coenzyme A from vitamin B5, where cysteine is conjugated to 4'-phosphopantothenate to form 4-phosphopantothenoylcysteine. It can be exsit as a homodimer(PMID:12906824).

Protocols

Product Specific Protocols
WB protocol for PPCS antibody 18001-1-APDownload protocol
IHC protocol for PPCS antibody 18001-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Int J Mol Sci

Regulation of the CoA Biosynthetic Complex Assembly in Mammalian Cells.

Authors - Jovana Baković
View Article