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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PPFIA3 Recombinant antibody, PBS Only (Capture)

PPFIA3 Recombinant Antibody for WB, IP, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230459F6

Cat no : 83142-4-PBS

Synonyms

PTPRF-interacting protein alpha-3, Protein tyrosine phosphatase receptor type f polypeptide-interacting protein alpha-3, Liprin-alpha-3, KIAA0654, 230459F6

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PPFIA3 Recombinant antibody (83142-4-RR)

    WB analysis using 83142-4-RR (same clone as 83142-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83142-4-RR (PPFIA3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83142-4-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using PPFIA3 Recombinant antibody (83142-4-RR)

    IP experiment of mouse brain using 83142-4-RR (same clone as 83142-4-PBS)

    IP result of anti-PPFIA3 (IP:83142-4-RR, 4ug; Detection:83142-4-RR 1:4000) with mouse brain tissue lysate 2100 ug. This data was developed using the same antibody clone with 83142-4-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HEK-293 cells using PPFIA3 Recombinant antibody (83142-4-RR)

    FC experiment of HEK-293 using 83142-4-RR (same clone as 83142-4-PBS)

    1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug PPFIA3 Recombinant antibody (83142-4-RR, Clone:230459F6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83142-4-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of U-251 cells using PPFIA3 Recombinant antibody (83142-4-RR)

    FC experiment of U-251 using 83142-4-RR (same clone as 83142-4-PBS)

    1x10^6 U-251 cells were intracellularly stained with 0.25 ug PPFIA3 Recombinant antibody (83142-4-RR, Clone:230459F6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83142-4-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00170-2

    Cytometric bead array standard curve of MP00170-2

    Cytometric bead array standard curve ofMP00170-2, PPFIA3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83142-4-PBS. Detection antibody: 83142-3-PBS. Standard: Ag1971. Range: 1.25-20 ng/mL.

  • Cytometric bead array standard curve of MP00170-3

    Cytometric bead array standard curve of MP00170-3

    Cytometric bead array standard curve ofMP00170-3, PPFIA3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83142-4-PBS. Detection antibody: 83142-2-PBS. Standard: Ag1971. Range: 1.25-20 ng/mL.

  • Affinity and Kinetic Characterization of 83142-4-RR

    Affinity and Kinetic Characterization of 83142-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83142-4-RR against Human PPFIA3 were performed. The affinity constant is 0.131 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83142-4-PBS targets PPFIA3 as part of a matched antibody pair:

MP00170-2: 83142-4-PBS capture and 83142-3-PBS detection (validated in Cytometric bead array)

MP00170-3: 83142-4-PBS capture and 83142-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PPFIA3 fusion protein Ag1971 相同性解析による交差性が予測される生物種
Full Name protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 3
Calculated molecular weight 1194 aa, 133 kDa
GenBank accession numberBC021255
Gene symbol PPFIA3
Gene ID (NCBI) 8541
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.