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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PPFIBP1 Polyclonal antibody

PPFIBP1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 13961-1-AP

Synonyms

PTPRF-interacting protein-binding protein 1, Protein tyrosine phosphatase receptor type f polypeptide-interacting protein-binding protein 1, Liprin-beta-1, KIAA1230, hSGT2

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using PPFIBP1 Polyclonal antibody (13961-1-AP)

    WB analysis of HepG2 using 13961-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 13961-1-AP (PPFIBP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of DU 145 cells using PPFIBP1 Polyclonal antibody (13961-1-AP)

    WB analysis of DU 145 using 13961-1-AP

    DU 145 cells were subjected to SDS PAGE followed by western blot with 13961-1-AP (PPFIBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using PPFIBP1 Polyclonal antibody (13961-1-AP)

    IP experiment of HepG2 using 13961-1-AP

    IP result of anti-PPFIBP1 (IP:13961-1-AP, 4ug; Detection:13961-1-AP 1:500) with HepG2 cells lysate 2400ug.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using PPFIBP1 Polyclonal antibody (13961-1-AP)

    IF Staining of U2OS using 13961-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using PPFIBP1 antibody (13961-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

Positive WB detected inHepG2 cells, DU 145 cells
Positive IP detected inHepG2 cells
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

13961-1-AP targets PPFIBP1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PPFIBP1 fusion protein Ag4884 相同性解析による交差性が予測される生物種
Full Name PTPRF interacting protein, binding protein 1 (liprin beta 1)
Calculated molecular weight 1011 aa, 114 kDa
Observed molecular weight 114 kDa
GenBank accession numberBC050281
Gene symbol PPFIBP1
Gene ID (NCBI) 8496
RRIDAB_2171631
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PPFIBP1 antibody 13961-1-APDownload protocol
IF protocol for PPFIBP1 antibody 13961-1-APDownload protocol
IP protocol for PPFIBP1 antibody 13961-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Open Biol

The structural basis of the talin-KANK1 interaction that coordinates the actin and microtubule cytoskeletons at focal adhesions

Authors - Xingchen Li
View Article
human

J Inflamm Res

Development and Validation of Diagnostic Models for Transcriptomic Signature Genes for Multiple Tissues in Osteoarthritis

Authors - Qichang Gao
View Article