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  • Featured Product
  • KD/KO Validated

PPFIBP2 Polyclonal antibody

PPFIBP2 Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 13953-1-AP

Synonyms

PTPRF-interacting protein-binding protein 2, Protein tyrosine phosphatase receptor type f polypeptide-interacting protein-binding protein 2, Liprin-beta-2

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human stomach tissue using PPFIBP2 Polyclonal antibody (13953-1-AP)

    IHC staining of human stomach using 13953-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13953-1-AP (PPFIBP2 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human stomach tissue using PPFIBP2 Polyclonal antibody (13953-1-AP)

    IHC staining of human stomach using 13953-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13953-1-AP (PPFIBP2 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using PPFIBP2 Polyclonal antibody (13953-1-AP)

    IF Staining of A431 using 13953-1-AP

    Immunofluorescent analysis of A431 cells using 13953-1-AP (PPFIBP2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13953-1-AP targets PPFIBP2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PPFIBP2 fusion protein Ag5064 相同性解析による交差性が予測される生物種
Full Name PTPRF interacting protein, binding protein 2 (liprin beta 2)
Calculated molecular weight 99 kDa
GenBank accession numberBC021714
Gene symbol PPFIBP2
Gene ID (NCBI) 8495
RRIDAB_2877997
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for PPFIBP2 antibody 13953-1-APDownload protocol
IF protocol for PPFIBP2 antibody 13953-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Ecotoxicol Environ Saf

Proteomic analysis reveals that cigarette smoke exposure diminishes ovarian reserve in mice by disrupting the CREB1-mediated ovarian granulosa cell proliferation-apoptosis balance

Authors - Mengting Xu
View Article
humanWB,IF

Biol Cell

Effects of the scaffold proteins liprin-α1, β1 and β2 on invasion by breast cancer cells.

Authors - Sara Chiaretti
  • KD Validated
View Article