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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PPID Monoclonal antibody

PPID Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1A2B1

Cat no : 67632-1-Ig

Synonyms

Cyclophilin-related protein, Cyclophilin-40, Cyclophilin D, Cyclophilin 40, 40 kDa peptidyl-prolyl cis-trans isomerase

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PPID Monoclonal antibody (67632-1-Ig)

    WB analysis using 67632-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67632-1-Ig (PPID antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using PPID Monoclonal antibody (67632-1-Ig)

    IHC staining of human cervical cancer using 67632-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67632-1-Ig (PPID antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using PPID Monoclonal antibody (67632-1-Ig)

    IHC staining of human cervical cancer using 67632-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67632-1-Ig (PPID antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inRaji cells, Jurkat cells, K-562 cells, JAR cells, pig brain issue, rat brain tissue, mouse brain tissue
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67632-1-Ig targets PPID in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PPID fusion protein Ag4371 相同性解析による交差性が予測される生物種
Full Name peptidylprolyl isomerase D
Calculated molecular weight 370 aa, 41 kDa
Observed molecular weight 38-41 kDa
GenBank accession numberBC030707
Gene symbol PPID
Gene ID (NCBI) 5481
RRIDAB_2882833
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PPID(Peptidyl-prolyl cis-trans isomerase D) is also named CYP40, CYPD, and belongs to the cyclophilin-type PPIase family, which accelerates the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. The inactivation of the Ppid gene rescued the disease phenotype of Col6a1 deficiency (PMID:19293339). This PPID antibody detected a strong band at an apparent molecular mass of 41 kDa and a weak band at 70 kDa due to crosslink (PMID: 33320090, 29097228).

Protocols

Product Specific Protocols
WB protocol for PPID antibody 67632-1-IgDownload protocol
IHC protocol for PPID antibody 67632-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols