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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PPID Recombinant antibody

PPID Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241361E4

Cat no : 84115-6-RR

Synonyms

Cyclophilin-related protein, Cyclophilin-40, Cyclophilin D, Cyclophilin 40, 40 kDa peptidyl-prolyl cis-trans isomerase

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PPID Recombinant antibody (84115-6-RR)

    WB analysis using 84115-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84115-6-RR (PPID antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of HepG2 cells using PPID Recombinant antibody (84115-6-RR)

    FC experiment of HepG2 using 84115-6-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug PPID Recombinant antibody (84115-6-RR, Clone:241361E4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 84115-6-RR

    Affinity and Kinetic Characterization of 84115-6-RR

    Biolayer interferometry (BLl) kinetic assays of 84115-6-RR against Human PPID were performed. The affinity constant is 0.497 nM.

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Tested Applications

Positive WB detected inA549 cells, MCF-7 cells, Jurkat cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84115-6-RR is PPID Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PPID fusion protein Ag3409 相同性解析による交差性が予測される生物種
Full Name peptidylprolyl isomerase D
Calculated molecular weight 370 aa, 41 kDa
Observed molecular weight41 kDa
GenBank accession numberBC030707
Gene symbol PPID
Gene ID (NCBI) 5481
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PPID(Peptidyl-prolyl cis-trans isomerase D) is also named CYP40, CYPD, and belongs to the cyclophilin-type PPIase family, which accelerates the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. The inactivation of the Ppid gene rescued the disease phenotype of Col6a1 deficiency (PMID:19293339). This PPID antibody detected a strong band at an apparent molecular mass of 41 kDa and a weak band at 70 kDa due to crosslink (PMID: 33320090, 29097228).

Protocols

Product Specific Protocols
WB protocol for PPID antibody 84115-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols