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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PPIG Polyclonal antibody

PPIG Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 12985-1-AP

Synonyms

Cyclophilin G, Clk-associating RS-cyclophilin, CASP10, CARS-Cyp, CARS-cyclophilin

Validation Data Gallery

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  • Western Blot (WB) analysis of human liver tissue using PPIG Polyclonal antibody (12985-1-AP)

    WB analysis of human liver using 12985-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 12985-1-AP (PPIG antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SH-SY5Y cells using PPIG Polyclonal antibody (12985-1-AP)

    WB analysis of SH-SY5Y using 12985-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 12985-1-AP (PPIG antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using PPIG Polyclonal antibody (12985-1-AP)

    WB analysis of NIH/3T3 using 12985-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12985-1-AP (PPIG antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using PPIG Polyclonal antibody (12985-1-AP)

    WB analysis of Jurkat using 12985-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 12985-1-AP (PPIG antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using PPIG Polyclonal antibody (12985-1-AP)

    WB analysis of Jurkat using 12985-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 12985-1-AP (PPIG antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SH-SY5Y cells using PPIG Polyclonal antibody (12985-1-AP)

    WB analysis of SH-SY5Y using 12985-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 12985-1-AP (PPIG antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using PPIG Polyclonal antibody (12985-1-AP)

    IHC staining of human prostate cancer using 12985-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12985-1-AP (PPIG antibody) at dilution of 1:100 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PPIG Polyclonal antibody (12985-1-AP)

    IF Staining of HeLa using 12985-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 12985-1-AP (PPIG antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PPIG Polyclonal antibody (12985-1-AP)

    IF Staining of HeLa using 12985-1-AP

    Immunofluorescent analysis of Hela cells, using PPIG antibody 12985-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG (green). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PPIG Polyclonal antibody (12985-1-AP)

    IF Staining of HeLa using 12985-1-AP

    Immunofluorescent analysis of Hela cells, using PPIG antibody 12985-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

Positive WB detected inhuman liver tissue, Jurkat cells, NIH/3T3 cells, SH-SY5Y cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

12985-1-AP targets PPIG in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PPIG fusion protein Ag3622 相同性解析による交差性が予測される生物種
Full Name peptidylprolyl isomerase G (cyclophilin G)
Calculated molecular weight 739 aa, 87 kDa
Observed molecular weight 89 kDa
GenBank accession numberBC001555
Gene symbol PPIG
Gene ID (NCBI) 9360
RRIDAB_2169281
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PPIG antibody 12985-1-APDownload protocol
IHC protocol for PPIG antibody 12985-1-APDownload protocol
IF protocol for PPIG antibody 12985-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Sci Adv

High-throughput tagging of endogenous loci for rapid characterization of protein function

Authors - Joonwon Kim
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Tatyana (Verified Customer) (12-22-2024)

ICC with antibody diluted in 5% goat serum in PBST, with 2 h incubation at RT. Good nuclear staining

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HeLa/human