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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PPIL3 Polyclonal antibody

PPIL3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15671-1-AP

Synonyms

Cyclophilin J, Cyclophilin like protein PPIL3, CYPJ, PPIase, PPIL3, Rotamase PPIL3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PPIL3 Polyclonal antibody (15671-1-AP)

    WB analysis using 15671-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15671-1-AP (PPIL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using PPIL3 Polyclonal antibody (15671-1-AP)

    IHC staining of human kidney using 15671-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15671-1-AP (PPIL3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using PPIL3 Polyclonal antibody (15671-1-AP)

    IHC staining of human kidney using 15671-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15671-1-AP (PPIL3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using PPIL3 Polyclonal antibody (15671-1-AP)

    IHC staining of human gliomas using 15671-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 15671-1-AP (PPIL3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using PPIL3 Polyclonal antibody (15671-1-AP)

    IHC staining of human gliomas using 15671-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 15671-1-AP (PPIL3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHCT 116 cells, mouse brain tissue, mouse liver tissue
Positive IHC detected inhuman kidney tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

15671-1-AP targets PPIL3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityrat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PPIL3 fusion protein Ag8137 相同性解析による交差性が予測される生物種
Full Name peptidylprolyl isomerase (cyclophilin)-like 3
Calculated molecular weight 161 aa, 18 kDa
GenBank accession numberBC007693
Gene symbol PPIL3
Gene ID (NCBI) 53938
RRIDAB_2169615
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PPIL3 antibody 15671-1-APDownload protocol
IHC protocol for PPIL3 antibody 15671-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratIHC

BMC Pregnancy Childbirth

Core biomarkers analysis benefit for diagnosis on human intrahepatic cholestasis of pregnancy

Authors - Yan Fang
View Article