• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PPIL4 Polyclonal antibody

PPIL4 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 12538-1-AP

Synonyms

PPIase, Peptidyl-prolyl cis-trans isomerase-like 4, HDCME13P, EC:5.2.1.8, Cyclophilin-like protein PPIL4

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using PPIL4 Polyclonal antibody (12538-1-AP)

    WB analysis using 12538-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12538-1-AP (PPIL4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PPIL4 Polyclonal antibody (12538-1-AP)

    WB analysis using 12538-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12538-1-AP (PPIL4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using PPIL4 Polyclonal antibody (12538-1-AP)

    WB analysis of HEK-293 using 12538-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 12538-1-AP (PPIL4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using PPIL4 Polyclonal antibody (12538-1-AP)

    WB analysis of HEK-293 using 12538-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 12538-1-AP (PPIL4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using PPIL4 Polyclonal antibody (12538-1-AP)

    WB analysis of mouse kidney using 12538-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12538-1-AP (PPIL4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using PPIL4 Polyclonal antibody (12538-1-AP)

    WB analysis of HeLa using 12538-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12538-1-AP (PPIL4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using PPIL4 Polyclonal antibody (12538-1-AP)

    WB analysis of HEK-293 using 12538-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 12538-1-AP (PPIL4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using PPIL4 Polyclonal antibody (12538-1-AP)

    IP experiment of HeLa using 12538-1-AP

    IP result of anti-PPIL4 (IP:12538-1-AP, 4ug; Detection:12538-1-AP 1:300) with HeLa cells lysate 2600 ug.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PPIL4 Polyclonal antibody (12538-1-AP)

    IF Staining of HeLa using 12538-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PPIL4 antibody (12538-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

☆大好評トライアルサイズ ¥20,000～！ >>> 一次抗体特別価格キャンペーン開催中！

Tested Applications

Positive WB detected inHEK-293 cells, HCT 116 cells, mouse kidney tissue, HeLa cells, HepG2 cells
Positive IP detected inHeLa cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

12538-1-AP targets PPIL4 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PPIL4 fusion protein Ag3249 相同性解析による交差性が予測される生物種
Full Name peptidylprolyl isomerase (cyclophilin)-like 4
Calculated molecular weight 492 aa, 57 kDa
Observed molecular weight 58-65 kDa
GenBank accession numberBC016984
Gene symbol PPIL4
Gene ID (NCBI) 85313
RRIDAB_10644441
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PPIL4 antibody 12538-1-APDownload protocol
IF protocol for PPIL4 antibody 12538-1-APDownload protocol
IP protocol for PPIL4 antibody 12538-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Int J Biol Sci

Up-regulation of RNA Binding Proteins Contributes to Folate Deficiency-Induced Neural Crest Cells Dysfunction.

Authors - Wenbo Liu
View Article