PPM1A Polyclonal antibody

Name: PPM1A Polyclonal antibody
Brand: Proteintech
SKU: 12961-1-AP

PPM1A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA, Cell treatment

Conjugate

Unconjugated

Publications

Review Product

Cat no : 12961-1-AP

Synonyms

PP2C ALPHA, PP2CA, PPM1A, PPPM1A, Protein phosphatase 1A, Protein phosphatase IA

Validation Data Gallery

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AllWBIHC

WB analysis using 12961-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12961-1-AP (PPM1A antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis using 12961-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12961-1-AP (PPM1A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 12961-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 12961-1-AP (PPM1A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of human liver using 12961-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 12961-1-AP (PPM1A antibody) at dilution of 1:900 incubated at room temperature for 1.5 hours.
IHC staining of human breast cancer using 12961-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12961-1-AP (PPM1A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human urothelial carcinoma using 12961-1-AP
Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 12961-1-AP (PPM1A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human urothelial carcinoma using 12961-1-AP
Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 12961-1-AP (PPM1A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 12961-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12961-1-AP (PPM1A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Cat No. 12961-1-AP

Size/Concentration

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	Jurkat cells, HepG2 cells, human liver tissue, rat kidney tissue, mouse kidney tissue, K-562 cells
Positive IHC detected in	human breast cancer tissue, human urothelial carcinoma tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 1 publications below
IF	See 1 publications below

Product Information

12961-1-AP targets PPM1A in WB, IF, IHC, ELISA, Cell treatment applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PPM1A fusion protein Ag3645 相同性解析による交差性が予測される生物種
Full Name	protein phosphatase 1A (formerly 2C), magnesium-dependent, alpha isoform
Calculated molecular weight	382 aa, 42 kDa
Observed molecular weight	42 kDa
GenBank accession number	BC026691
Gene symbol	PPM1A
Gene ID (NCBI)	5494
RRID	AB_2169763
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PPM1A(Protein phosphatase 1A) is also named as PPPM1A and belongs to the PP2C family. It is a serine/threonine protein phosphatase that is essential for regulating cellular stress responses in eukaryotes. PPM1A plays an important role in tumor suppression and that it negatively controls many signaling pathways which are critical for trophoblast invasion.(PMID:19404668). This protein distributes in both the cytoplasm and nucleus of mammalian cells(PMID:9003755). It has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for PPM1A antibody 12961-1-AP	Download protocol
IHC protocol for PPM1A antibody 12961-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	Cell treatment	Cell Rep Blocking AMPK β1 myristoylation enhances AMPK activity and protects mice from high-fat diet-induced obesity and hepatic steatosis Authors - Katyayanee Neopane View Article
rat	IF	iScience Induced retinal pigment epithelial cells with anti-epithelial-to-mesenchymal transition ability delay retinal degeneration Authors - Haibin Tian View Article
mouse	WB	Biol Pharm Bull Sorghum (Sorghum bicolor) Extract-Induced Adipogenesis Is Independent of PPARγ Ser273 Phosphorylation in 3T3-L1 Adipocytes. Authors - Aozora Nagaoka View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PPM1A Polyclonal antibody

PPM1A Polyclonal Antibody for WB, IHC, ELISA

Validation Data Gallery

WB analysis using 12961-1-AP

WB analysis using 12961-1-AP

WB analysis of HepG2 using 12961-1-AP

WB analysis of human liver using 12961-1-AP

IHC staining of human breast cancer using 12961-1-AP

IHC staining of human urothelial carcinoma using 12961-1-AP