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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PPM1B Monoclonal antibody, PBS Only

PPM1B Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2C7H2

Cat no : 67647-1-PBS

Synonyms

PP2C beta, PP2C beta X, PP2CB, PP2CBETA, PPC2BETAX, PPM1B, Protein phosphatase 1B

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PPM1B Monoclonal antibody (67647-1-Ig)

    WB analysis using 67647-1-Ig (same clone as 67647-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67647-1-Ig (PPM1B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67647-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PPM1B Monoclonal antibody (67647-1-Ig)

    WB analysis using 67647-1-Ig (same clone as 67647-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67647-1-Ig (PPM1B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67647-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PPM1B Monoclonal antibody (67647-1-Ig)

    IHC staining of human breast cancer using 67647-1-Ig (same clone as 67647-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67647-1-Ig (PPM1B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67647-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PPM1B Monoclonal antibody (67647-1-Ig)

    IHC staining of human breast cancer using 67647-1-Ig (same clone as 67647-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67647-1-Ig (PPM1B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67647-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using PPM1B Monoclonal antibody (67647-1-Ig)

    IHC staining of mouse skeletal muscle using 67647-1-Ig (same clone as 67647-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67647-1-Ig (PPM1B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67647-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using PPM1B Monoclonal antibody (67647-1-Ig)

    IHC staining of mouse skeletal muscle using 67647-1-Ig (same clone as 67647-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67647-1-Ig (PPM1B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67647-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67647-1-PBS targets PPM1B in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PPM1B fusion protein Ag3871 相同性解析による交差性が予測される生物種
Full Name protein phosphatase 1B (formerly 2C), magnesium-dependent, beta isoform
Calculated molecular weight 479 aa, 53 kDa
Observed molecular weight 36, 53 kDa
GenBank accession numberBC012002
Gene symbol PPM1B
Gene ID (NCBI) 5495
RRIDAB_2882847
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PPM1B can identify 4 isoforms with the WM of 36-55 kDa.