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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PPM1B Monoclonal antibody (67647-1-Ig)

    WB analysis using 67647-1-Ig (same clone as 67647-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67647-1-Ig (PPM1B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67647-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PPM1B Monoclonal antibody (67647-1-Ig)

    WB analysis using 67647-1-Ig (same clone as 67647-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67647-1-Ig (PPM1B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67647-1-PBS in a different storage buffer formulation.

  • Sample test of MP51322-1

    Sample test of MP51322-1

    Sample test of MP51322-1, PPM1B Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67647-1-PBS. Detection antibody: 67647-2-PBS.

  • Sample test of MP51322-2

    Sample test of MP51322-2

    Sample test of MP51322-2, PPM1B Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67647-1-PBS. Detection antibody: 67647-3-PBS.

  • Sample test of MP51322-3

    Sample test of MP51322-3

    Sample test of MP51322-3, PPM1B Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67647-1-PBS. Detection antibody: 67647-4-PBS.

  • Sample test of MP51322-4

    Sample test of MP51322-4

    Sample test of MP51322-4, PPM1B Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67647-1-PBS. Detection antibody: 67647-5-PBS.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PPM1B Monoclonal antibody (67647-1-Ig)

    IHC staining of human breast cancer using 67647-1-Ig (same clone as 67647-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67647-1-Ig (PPM1B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67647-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PPM1B Monoclonal antibody (67647-1-Ig)

    IHC staining of human breast cancer using 67647-1-Ig (same clone as 67647-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67647-1-Ig (PPM1B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67647-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using PPM1B Monoclonal antibody (67647-1-Ig)

    IHC staining of mouse skeletal muscle using 67647-1-Ig (same clone as 67647-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67647-1-Ig (PPM1B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67647-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using PPM1B Monoclonal antibody (67647-1-Ig)

    IHC staining of mouse skeletal muscle using 67647-1-Ig (same clone as 67647-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67647-1-Ig (PPM1B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67647-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using PPM1B Monoclonal antibody (67647-1-Ig)

    IF Staining of A431 using 67647-1-Ig (same clone as 67647-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using PPM1B antibody (67647-1-Ig, Clone: 2C7H2 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67647-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP51322-1

    Cytometric bead array standard curve of MP51322-1

    Cytometric bead array standard curve of MP51322-1, PPM1B Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67647-1-PBS. Detection antibody: 67647-2-PBS. Standard:Ag3871. Range: 0.195-200 ng/mL.

  • Cytometric bead array standard curve of MP51322-2

    Cytometric bead array standard curve of MP51322-2

    Cytometric bead array standard curve of MP51322-2, PPM1B Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67647-1-PBS. Detection antibody: 67647-3-PBS. Standard:Ag3871. Range: 0.195-200 ng/mL.

  • Cytometric bead array standard curve of MP51322-3

    Cytometric bead array standard curve of MP51322-3

    Cytometric bead array standard curve of MP51322-3, PPM1B Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67647-1-PBS. Detection antibody: 67647-4-PBS. Standard:Ag3871. Range: 0.195-200 ng/mL.

  • Cytometric bead array standard curve of MP51322-4

    Cytometric bead array standard curve of MP51322-4

    Cytometric bead array standard curve of MP51322-4, PPM1B Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67647-1-PBS. Detection antibody: 67647-5-PBS. Standard:Ag3871. Range: 0.195-200 ng/mL.

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PPM1B Monoclonal antibody, PBS Only (Capture)

PPM1B Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA, Sample test
Cat No. 67647-1-PBS
Clone No.2C7H2

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA, Sample test

Synonyms

2C7H2, EC:3.1.3.16, PP2C beta, PP2CB, PP2C-beta

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67647-1-PBS targets PPM1B as part of a matched antibody pair:

MP51322-1: 67647-1-PBS capture and 67647-2-PBS detection (validated in Cytometric bead array)

MP51322-2: 67647-1-PBS capture and 67647-3-PBS detection (validated in Cytometric bead array)

MP51322-3: 67647-1-PBS capture and 67647-4-PBS detection (validated in Cytometric bead array)

MP51322-4: 67647-1-PBS capture and 67647-5-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PPM1B fusion protein Ag3871 相同性解析による交差性が予測される生物種
Full Name protein phosphatase 1B (formerly 2C), magnesium-dependent, beta isoform
Calculated molecular weight 479 aa, 53 kDa
Observed molecular weight 36, 53 kDa
GenBank accession numberBC012002
Gene Symbol PPM1B
Gene ID (NCBI) 5495
RRIDAB_2882847
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO75688
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PPM1B can identify 4 isoforms with the WM of 36-55 kDa.