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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PPM1B Monoclonal antibody, PBS Only

PPM1B Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2C7H2

Cat no : 67647-1-PBS

Synonyms

PP2C beta, PP2C beta X, PP2CB, PP2CBETA, PPC2BETAX, PPM1B, Protein phosphatase 1B

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PPM1B Monoclonal antibody (67647-1-Ig)

    WB analysis using 67647-1-Ig (same clone as 67647-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67647-1-Ig (PPM1B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67647-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PPM1B Monoclonal antibody (67647-1-Ig)

    WB analysis using 67647-1-Ig (same clone as 67647-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67647-1-Ig (PPM1B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67647-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PPM1B Monoclonal antibody (67647-1-Ig)

    IHC staining of human breast cancer using 67647-1-Ig (same clone as 67647-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67647-1-Ig (PPM1B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67647-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PPM1B Monoclonal antibody (67647-1-Ig)

    IHC staining of human breast cancer using 67647-1-Ig (same clone as 67647-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67647-1-Ig (PPM1B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67647-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using PPM1B Monoclonal antibody (67647-1-Ig)

    IHC staining of mouse skeletal muscle using 67647-1-Ig (same clone as 67647-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67647-1-Ig (PPM1B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67647-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using PPM1B Monoclonal antibody (67647-1-Ig)

    IHC staining of mouse skeletal muscle using 67647-1-Ig (same clone as 67647-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67647-1-Ig (PPM1B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67647-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67647-1-PBS targets PPM1B in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PPM1B fusion protein Ag3871 相同性解析による交差性が予測される生物種
Full Name protein phosphatase 1B (formerly 2C), magnesium-dependent, beta isoform
Calculated molecular weight 479 aa, 53 kDa
Observed molecular weight 36, 53 kDa
GenBank accession numberBC012002
Gene symbol PPM1B
Gene ID (NCBI) 5495
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PPM1B can identify 4 isoforms with the WM of 36-55 kDa.