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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PPM1D Polyclonal antibody

PPM1D Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, Rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 26532-1-AP

Synonyms

PP2C DELTA, PPM1D, Protein phosphatase 1D, WIP1

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using PPM1D Polyclonal antibody (26532-1-AP)

    WB analysis of MCF-7 using 26532-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 26532-1-AP (PPM1D antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using PPM1D Polyclonal antibody (26532-1-AP)

    WB analysis of mouse lung using 26532-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 26532-1-AP (PPM1D Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat testis tissue using PPM1D Polyclonal antibody (26532-1-AP)

    WB analysis of rat testis using 26532-1-AP

    rat testis tissue were subjected to SDS PAGE followed by western blot with 26532-1-AP (PPM1D Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using PPM1D Polyclonal antibody (26532-1-AP)

    WB analysis of MCF-7 using 26532-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 26532-1-AP (PPM1D antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using PPM1D Polyclonal antibody (26532-1-AP)

    IF Staining of MCF-7 using 26532-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 26532-1-AP (PPM1D antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using PPM1D Polyclonal antibody (26532-1-AP)

    IF Staining of MCF-7 using 26532-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 26532-1-AP (PPM1D antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inMCF-7 cells, mouse lung tissue, rat testis tissue
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 26532-1-AP is PPM1D Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, Rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PPM1D fusion protein Ag24751 相同性解析による交差性が予測される生物種
Full Name protein phosphatase 1D magnesium-dependent, delta isoform
Observed molecular weight 67 kDa
GenBank accession numberBC060877
Gene symbol PPM1D
Gene ID (NCBI) 8493
RRIDAB_2880544
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PPM1D (also known as PP2Cd and WIP1) is a member of the PPM family, which is characterized by Mg2+/Mn2+-dependent activity and relative insensitivity to okadaic acid. The PPM1D gene is aberrantly amplified in a range of common cancers and encodes a protein phosphatase that is a potential therapeutic target (PMID: 17700519). This antibody can be detected two bands of 47 and 67 Kda.

Protocols

Product Specific Protocols
WB protocol for PPM1D antibody 26532-1-APDownload protocol
IF protocol for PPM1D antibody 26532-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols