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Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using PPM1D Monoclonal antibody (67440-1-Ig)

    WB analysis of MCF-7 using 67440-1-Ig

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 67440-1-Ig (PPM1D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig brain tissue using PPM1D Monoclonal antibody (67440-1-Ig)

    WB analysis of pig brain using 67440-1-Ig

    pig brain tissue were subjected to SDS PAGE followed by western blot with 67440-1-Ig (PPM1D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using PPM1D Monoclonal antibody (67440-1-Ig)

    WB analysis of HEK-293 using 67440-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67440-1-Ig (PPM1D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of U2OS cells using PPM1D Monoclonal antibody (67440-1-Ig)

    WB analysis of U2OS using 67440-1-Ig

    U2OS cells were subjected to SDS PAGE followed by western blot with 67440-1-Ig (PPM1D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using PPM1D Monoclonal antibody (67440-1-Ig)

    WB analysis of rat brain using 67440-1-Ig

    rat brain tissue were subjected to SDS PAGE followed by western blot with 67440-1-Ig (PPM1D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using PPM1D Monoclonal antibody (67440-1-Ig)

    WB analysis of mouse brain using 67440-1-Ig

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 67440-1-Ig (PPM1D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PPM1D Monoclonal antibody (67440-1-Ig)

    IHC staining of human colon cancer using 67440-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67440-1-Ig (PPM1D antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PPM1D Monoclonal antibody (67440-1-Ig)

    IHC staining of human colon cancer using 67440-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67440-1-Ig (PPM1D antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PPM1D Monoclonal antibody (67440-1-Ig)

    IF Staining of HepG2 using 67440-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 67440-1-Ig (PPM1D antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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PPM1D Monoclonal antibody

PPM1D Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67440-1-Ig
Clone No.2D6D5

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

PP2C DELTA, PPM1D, Protein phosphatase 1D, WIP1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMCF-7 cells, HEK-293 cells, U2OS cells, pig brain tissue, rat brain tissue, mouse brain tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67440-1-Ig targets PPM1D in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Reactivity Human, Mouse, Rat, Pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PPM1D fusion protein Ag24761 相同性解析による交差性が予測される生物種
Full Name protein phosphatase 1D magnesium-dependent, delta isoform
Observed molecular weight 67 kDa, 47 kDa
GenBank accession numberBC060877
Gene Symbol PPM1D
Gene ID (NCBI) 8493
RRIDAB_2882676
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO15297
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PPM1D (also known as PP2Cd and WIP1) is a member of the PPM family, which is characterized by Mg2+/Mn2+-dependent activity and relative insensitivity to okadaic acid. The PPM1D gene is aberrantly amplified in a range of common cancers and encodes a protein phosphatase that is a potential therapeutic target (PMID: 17700519). This antibody can be detected two bands of 47 and 67 Kda.

Protocols

Product Specific Protocols
WB protocol for PPM1D antibody 67440-1-IgDownload protocol
IHC protocol for PPM1D antibody 67440-1-IgDownload protocol
IF protocol for PPM1D antibody 67440-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols