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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PPM1E Polyclonal antibody (25089-1-AP)

    WB analysis using 25089-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25089-1-AP (PPM1E antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of rat brain tissue using PPM1E Polyclonal antibody (25089-1-AP)

    IHC staining of rat brain using 25089-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 25089-1-AP (PPM1E antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using PPM1E Polyclonal antibody (25089-1-AP)

    IF Staining of A431 using 25089-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using PPM1E antibody (25089-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using PPM1E Polyclonal antibody (25089-1-AP)

    IF Staining of SH-SY5Y using 25089-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using PPM1E antibody (25089-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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PPM1E Polyclonal antibody

PPM1E Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 25089-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

EC:3.1.3.16, CaMKP-nucleus, CaMKP-N, CaMKP N, CaMKN

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman brain tissue, mouse brain tissue, rat brain tissue
Positive IHC detected inrat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells, A431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25089-1-AP targets PPM1E in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PPM1E fusion protein Ag18751 相同性解析による交差性が予測される生物種
Full Name protein phosphatase 1E (PP2C domain containing)
Calculated molecular weight 764 aa, 85 kDa
Observed molecular weight85 kDa
GenBank accession numberBC136290
Gene Symbol PPM1E
Gene ID (NCBI) 22843
RRIDAB_3085770
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WY54
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Ca2+/calmodulin-dependent protein kinase phosphatase (CaMKP-N),also known as Ppm1E, is an AMPKα phosphatase,which is up-regulated in human gastric cancer tissues (PMID: 28423719).

Protocols

Product Specific Protocols
WB protocol for PPM1E antibody 25089-1-APDownload protocol
IHC protocol for PPM1E antibody 25089-1-APDownload protocol
IF protocol for PPM1E antibody 25089-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols