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  • Featured Product
  • KD/KO Validated

PPM1F Polyclonal antibody

PPM1F Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 17020-1-AP

Synonyms

FEM-2, EC:3.1.3.16, CaMKPase, CaM-kinase phosphatase, CaM kinase phosphatase

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using PPM1F Polyclonal antibody (17020-1-AP)

    WB analysis of MCF-7 using 17020-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 17020-1-AP (PPM1F antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PPM1F Polyclonal antibody (17020-1-AP)

    WB analysis using 17020-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17020-1-AP (PPM1F antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using PPM1F Polyclonal antibody (17020-1-AP)

    WB analysis of HeLa using 17020-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17020-1-AP (PPM1F Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using PPM1F Polyclonal antibody (17020-1-AP)

    WB analysis of HepG2 using 17020-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 17020-1-AP (PPM1F antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using PPM1F Polyclonal antibody (17020-1-AP)

    WB analysis of L02 using 17020-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 17020-1-AP (PPM1F antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using PPM1F Polyclonal antibody (17020-1-AP)

    WB analysis of human liver using 17020-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 17020-1-AP (PPM1F antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using PPM1F Polyclonal antibody (17020-1-AP)

    WB analysis of K-562 using 17020-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 17020-1-AP (PPM1F antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of MCF-7 cells using PPM1F Polyclonal antibody (17020-1-AP)

    IP experiment of MCF-7 using 17020-1-AP

    IP result of anti-PPM1F (IP:17020-1-AP, 4ug; Detection:17020-1-AP 1:700) with MCF-7 cells lysate 2800ug.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PPM1F Polyclonal antibody (17020-1-AP)

    IHC staining of human colon cancer using 17020-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17020-1-AP (PPM1F antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using PPM1F Polyclonal antibody (17020-1-AP)

    IHC staining of human ovary cancer using 17020-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 17020-1-AP (PPM1F antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PPM1F Polyclonal antibody (17020-1-AP)

    IF Staining of HepG2 using 17020-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 17020-1-AP (PPM1F antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inMCF-7 cells, L02 cells, HepG2 cells, human liver tissue, K-562 cells, HeLa cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman ovary cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17020-1-AP targets PPM1F in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PPM1F fusion protein Ag10186 相同性解析による交差性が予測される生物種
Full Name protein phosphatase 1F (PP2C domain containing)
Calculated molecular weight 453 aa, 50 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC071989
Gene symbol PPM1F
Gene ID (NCBI) 9647
RRIDAB_2284338
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Mg2+/Mn2+-dependent protein phosphatase 1F (PPM1F) belongs to the PP2C family of Ser/Thr protein phosphatases and is the critical enzyme responsible for dephosphorylating the threonine motif. PPM1F is ubiquitous in various tissues and organs (PMID: 26498692). High expression of PPM1F was positively associated with poor survival and tumor recurrence in patients with cancers and PPM1F might be a potential biomarker in HCC patients (PMID: 30470261).

Protocols

Product Specific Protocols
WB protocol for PPM1F antibody 17020-1-APDownload protocol
IHC protocol for PPM1F antibody 17020-1-APDownload protocol
IF protocol for PPM1F antibody 17020-1-APDownload protocol
IP protocol for PPM1F antibody 17020-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Cell Biol

PPM1F controls integrin activity via a conserved phospho-switch.

Authors - Tanja M Grimm
  • KO Validated
View Article
humanWB

Clin Transl Oncol

PPM1F regulates ovarian cancer progression by affecting the dephosphorylation of ITGB1

Authors - Yahui Leng
View Article