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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PPM1F Monoclonal antibody (68626-1-Ig)

    WB analysis using 68626-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68626-1-Ig (PPM1F antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of various lysates using PPM1F Monoclonal antibody (68626-1-Ig)

    WB analysis using 68626-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68626-1-Ig (PPM1F antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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PPM1F Monoclonal antibody

PPM1F Monoclonal Antibody for WB, ELISA
Cat No. 68626-1-Ig
Clone No.2A2E8

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, ELISA

Synonyms

CaM kinase phosphatase, CaMKPase, FEM 2, hFEM 2, KIAA0015, Partner of PIX 2, POPX2, PPM1F, Protein fem 2 homolog, Protein phosphatase 1F

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inU2OS cells, A375 cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, human peripheral blood platelets

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68626-1-Ig targets PPM1F in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen PPM1F fusion protein Ag10316 相同性解析による交差性が予測される生物種
Full Name protein phosphatase 1F (PP2C domain containing)
Calculated molecular weight 453 aa, 50 kDa
Observed molecular weight50 kDa
GenBank accession numberBC071989
Gene Symbol PPM1F
Gene ID (NCBI) 9647
RRIDAB_3085318
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP49593
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Mg2+/Mn2+-dependent protein phosphatase 1F (PPM1F) belongs to the PP2C family of Ser/Thr protein phosphatases and is the critical enzyme responsible for dephosphorylating the threonine motif. PPM1F is ubiquitous in various tissues and organs (PMID: 26498692). High expression of PPM1F was positively associated with poor survival and tumor recurrence in patients with cancers and PPM1F might be a potential biomarker in HCC patients (PMID: 30470261).

Protocols

Product Specific Protocols
WB protocol for PPM1F antibody 68626-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols