Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis using 15532-1-AP (same clone as 15532-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 15532-1-AP (PPM1G antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15532-1-PBS in a different storage buffer formulation.
WB analysis of mouse lung using 15532-1-AP (same clone as 15532-1-PBS)
mouse lung tissue were subjected to SDS PAGE followed by western blot with 15532-1-AP (PPM1G antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15532-1-PBS in a different storage buffer formulation.
IHC staining of mouse testis using 15532-1-AP (same clone as 15532-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15532-1-AP (PPM1G antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15532-1-PBS in a different storage buffer formulation.
IHC staining of mouse kidney using 15532-1-AP (same clone as 15532-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15532-1-AP (PPM1G antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15532-1-PBS in a different storage buffer formulation.
IHC staining of mouse kidney using 15532-1-AP (same clone as 15532-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15532-1-AP (PPM1G antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15532-1-PBS in a different storage buffer formulation.
IHC staining of mouse testis using 15532-1-AP (same clone as 15532-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15532-1-AP (PPM1G antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15532-1-PBS in a different storage buffer formulation.
IHC staining of human placenta using 15532-1-AP (same clone as 15532-1-PBS)
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15532-1-AP (PPM1G antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15532-1-PBS in a different storage buffer formulation.
IHC staining of human placenta using 15532-1-AP (same clone as 15532-1-PBS)
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15532-1-AP (PPM1G antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15532-1-PBS in a different storage buffer formulation.
IHC staining of mouse spleen using 15532-1-AP (same clone as 15532-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 15532-1-AP (PPM1G antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15532-1-PBS in a different storage buffer formulation.
IHC staining of mouse spleen using 15532-1-AP (same clone as 15532-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 15532-1-AP (PPM1G antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15532-1-PBS in a different storage buffer formulation.
IHC staining of human heart using 15532-1-AP (same clone as 15532-1-PBS)
Immunohistochemical analysis of paraffin-embedded human heart using 15532-1-AP (PPM1G antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 15532-1-PBS in a different storage buffer formulation.
IHC staining of human heart using 15532-1-AP (same clone as 15532-1-PBS)
Immunohistochemical analysis of paraffin-embedded human heart using 15532-1-AP (PPM1G antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 15532-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 15532-1-AP (same clone as 15532-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney using 15532-1-AP (PPM1G antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 15532-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 15532-1-AP (same clone as 15532-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney using 15532-1-AP (PPM1G antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 15532-1-PBS in a different storage buffer formulation.
IHC staining of human skin using 15532-1-AP (same clone as 15532-1-PBS)
Immunohistochemical analysis of paraffin-embedded human skin using 15532-1-AP (PPM1G antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 15532-1-PBS in a different storage buffer formulation.
IHC staining of human skin using 15532-1-AP (same clone as 15532-1-PBS)
Immunohistochemical analysis of paraffin-embedded human skin using 15532-1-AP (PPM1G antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 15532-1-PBS in a different storage buffer formulation.
IHC staining of human brain using 15532-1-AP (same clone as 15532-1-PBS)
Immunohistochemical analysis of paraffin-embedded human brain using 15532-1-AP (PPM1G antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 15532-1-PBS in a different storage buffer formulation.
IHC staining of human brain using 15532-1-AP (same clone as 15532-1-PBS)
Immunohistochemical analysis of paraffin-embedded human brain using 15532-1-AP (PPM1G antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 15532-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 15532-1-AP (same clone as 15532-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver using 15532-1-AP (PPM1G antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 15532-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 15532-1-AP (same clone as 15532-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver using 15532-1-AP (PPM1G antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 15532-1-PBS in a different storage buffer formulation.
IF Staining of MCF-7 using 15532-1-AP (same clone as 15532-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PPM1G antibody (15532-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 15532-1-PBS in a different storage buffer formulation.
IF Staining of MCF-7 using 15532-1-AP (same clone as 15532-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PPM1G antibody (15532-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15532-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

PPM1G Polyclonal antibody, PBS Only

PPM1G Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 15532-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15532-1-PBS targets PPM1G in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PPM1G fusion protein Ag7751 相同性解析による交差性が予測される生物種
Full Name	protein phosphatase 1G (formerly 2C), magnesium-dependent, gamma isoform
Calculated molecular weight	59 kDa
Observed molecular weight	70 kDa
GenBank accession number	BC000057
Gene Symbol	PPM1G
Gene ID (NCBI)	5496
RRID	AB_2169934
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O15355
Storage Buffer	PBS only , pH 7.3
Storage Conditions	Store at -80°C.

Background Information

PPM1G, also known as PP2Cγ and PP2CG, is an important member of the PP2C family of serine/threonine protein phosphatases and has an important role in controlling cell cycle progression. PPM1G restricts innate immune signaling mediated by STING and MAVS and is hijacked by KSHV for immune evasio(PMID: 33219031). In addition, PPM1G promotes the progression of hepatocellular carcinoma via phosphorylation regulation of alternative splicing protein SRSF3(PMID: 34290239).

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 15532-1-AP (same clone as 15532-1-PBS)

WB analysis of mouse lung using 15532-1-AP (same clone as 15532-1-PBS)

IHC staining of mouse testis using 15532-1-AP (same clone as 15532-1-PBS)

IHC staining of mouse kidney using 15532-1-AP (same clone as 15532-1-PBS)

IHC staining of mouse kidney using 15532-1-AP (same clone as 15532-1-PBS)

IHC staining of mouse testis using 15532-1-AP (same clone as 15532-1-PBS)

IHC staining of human placenta using 15532-1-AP (same clone as 15532-1-PBS)

IHC staining of human placenta using 15532-1-AP (same clone as 15532-1-PBS)

IHC staining of mouse spleen using 15532-1-AP (same clone as 15532-1-PBS)