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Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using PPM1K Polyclonal antibody (14573-1-AP)

    WB analysis of human brain using 14573-1-AP (same clone as 14573-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 14573-1-AP (PPM1K antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14573-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human heart tissue using PPM1K Polyclonal antibody (14573-1-AP)

    WB analysis of human heart using 14573-1-AP (same clone as 14573-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 14573-1-AP (PPM1K antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14573-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using PPM1K Polyclonal antibody (14573-1-AP)

    IHC staining of human gliomas using 14573-1-AP (same clone as 14573-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas using 14573-1-AP (PPM1K antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14573-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using PPM1K Polyclonal antibody (14573-1-AP)

    IHC staining of human gliomas using 14573-1-AP (same clone as 14573-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas using 14573-1-AP (PPM1K antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14573-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

PPM1K Polyclonal antibody, PBS Only

PPM1K Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 14573-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14573-1-PBS targets PPM1K in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PPM1K fusion protein Ag5660 相同性解析による交差性が予測される生物種
Full Name protein phosphatase 1K (PP2C domain containing)
Calculated molecular weight 41 kDa
Observed molecular weight30-40 kDa
GenBank accession numberBC037552
Gene Symbol PPM1K
Gene ID (NCBI) 152926
RRIDAB_2252953
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N3J5
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Protein phosphatase, Mg2+/Mn2+ dependent 1K (PPM1K), is a member of the PPM family of Mn2+/Mg2+-dependent protein phosphatases. PPMiK, essential for cell survival and development, is targeted to the mitochondria where it plays a key role in regulation of the mitochondrial permeability transition pore.PPM1K is essential for the regulation and nutrient-induced activation of the branched-chain alpha-keto acid dehydrogenase complex (BCKD or BCKDC), which catalyzes the breakdown of branched-chain amino acids (BCAA).