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Validation Data Gallery

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  • Western Blot (WB) analysis of human lung tissue using PPM1M Polyclonal antibody (12104-1-AP)

    WB analysis of human lung using 12104-1-AP

    human lung tissue were subjected to SDS PAGE followed by western blot with 12104-1-AP (PPM1M antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse lung tissue using PPM1M Polyclonal antibody (12104-1-AP)

    IP experiment of mouse lung using 12104-1-AP

    IP result of anti-PPM1M (IP:12104-1-AP, 4ug; Detection:12104-1-AP 1:300) with mouse lung tissue lysate 4000ug.

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PPM1M Polyclonal antibody

PPM1M Polyclonal Antibody for WB, IP, ELISA
Cat No. 12104-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, ELISA

Synonyms

PP2C eta, PP2CE, PP2Ceta, PPM1E, PPM1M, Protein phosphatase 1M

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman lung tissue
Positive IP detected inmouse lung tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12104-1-AP targets PPM1M in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PPM1M fusion protein Ag2748 相同性解析による交差性が予測される生物種
Full Name protein phosphatase 1M (PP2C domain containing)
Calculated molecular weight 270 aa, 30 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC009644
Gene Symbol PPM1M
Gene ID (NCBI) 132160
RRIDAB_2170362
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96MI6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PPM1M antibody 12104-1-APDownload protocol
IP protocol for PPM1M antibody 12104-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols