Validation Data Gallery
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Tested Applications
| Positive WB detected in | PC-3 cells, mouse skeletal muscle tissue, mouse brain tissue, mouse heart tissue, mouse liver tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human prostate cancer tissue, human thyroid cancer tissue, mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 5 publications below |
| IHC | See 2 publications below |
Product Information
21176-1-AP targets PDP1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PDP1 fusion protein Ag15453 相同性解析による交差性が予測される生物種 |
| Full Name | protein phosphatase 2C, magnesium-dependent, catalytic subunit |
| Calculated molecular weight | 537 aa, 61 kDa |
| Observed molecular weight | 55-61 kDa |
| GenBank accession number | BC047619 |
| Gene Symbol | PDP1 |
| Gene ID (NCBI) | 54704 |
| RRID | AB_2878824 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9P0J1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Pyruvate dehydrogenase phosphatase 1 (PDP1) is a key hormone-regulated metabolic enzyme that dephosphorylates and activates pyruvate dehydrogenase (PDH), thereby stimulating the conversion of pyruvate into acetyl-CoA (PMID: 36453802). PDP1 expression is regulated by FLT3-ITD in a context-dependent manner, and that it is centrally involved in the adaptation of AML glucose metabolism to the requirements of proliferation, survival and the therapeutic response to FLT3 inhibition (PMID: 37935978).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PDP1 antibody 21176-1-AP | Download protocol |
| IHC protocol for PDP1 antibody 21176-1-AP | Download protocol |
| IP protocol for PDP1 antibody 21176-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Aging (Albany NY) Identification of immune-related cells and genes in the breast invasive carcinoma microenvironment. | ||
Curr Med Sci Salidroside Inhibits Lipopolysaccharide-ethanol-induced Activation of Proinflammatory Macrophages via Notch Signaling Pathway. | ||
J Diabetes Res Pyruvate-Enriched Oral Rehydration Solution Improves Glucometabolic Disorders in the Kidneys of Diabetic db/db Mice. | ||
Cell Death Dis Mitochondrial dysfunction induces radioresistance in colorectal cancer by activating [Ca2+]m-PDP1-PDH-histone acetylation retrograde signaling. | ||
ACS Chem Neurosci FDCA Attenuates Neuroinflammation and Brain Injury after Cerebral Ischemic Stroke | ||
J Physiol Monocarboxylate transporter 4 deficiency enhances high-intensity interval training-induced metabolic adaptations in skeletal muscle |