PDP1 Polyclonal antibody

PDP1 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 21176-1-AP

Synonyms

PPM2C, Protein phosphatase 2C, PDPC 1, PDP 1, EC:3.1.3.43


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Tested Applications

Positive WB detected inPC-3 cells, mouse skeletal muscle tissue, mouse brain tissue, mouse heart tissue, mouse liver tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman prostate cancer tissue, human thyroid cancer tissue, mouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21176-1-AP targets PDP1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PDP1 fusion protein Ag15453 相同性解析による交差性が予測される生物種
Full Name protein phosphatase 2C, magnesium-dependent, catalytic subunit
Calculated molecular weight 537 aa, 61 kDa
Observed molecular weight 55-61 kDa
GenBank accession numberBC047619
Gene symbol PDP1
Gene ID (NCBI) 54704
RRIDAB_2878824
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Pyruvate dehydrogenase phosphatase 1 (PDP1) is a key hormone-regulated metabolic enzyme that dephosphorylates and activates pyruvate dehydrogenase (PDH), thereby stimulating the conversion of pyruvate into acetyl-CoA (PMID: 36453802). PDP1 expression is regulated by FLT3-ITD in a context-dependent manner, and that it is centrally involved in the adaptation of AML glucose metabolism to the requirements of proliferation, survival and the therapeutic response to FLT3 inhibition (PMID: 37935978).

Protocols

Product Specific Protocols
WB protocol for PDP1 antibody 21176-1-APDownload protocol
IHC protocol for PDP1 antibody 21176-1-APDownload protocol
IP protocol for PDP1 antibody 21176-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Aging (Albany NY)

Identification of immune-related cells and genes in the breast invasive carcinoma microenvironment.

Authors - Xingfeng Chen
humanWB

Curr Med Sci

Salidroside Inhibits Lipopolysaccharide-ethanol-induced Activation of Proinflammatory Macrophages via Notch Signaling Pathway.

Authors - Jian-Sha Li
mouseWB

J Diabetes Res

Pyruvate-Enriched Oral Rehydration Solution Improves Glucometabolic Disorders in the Kidneys of Diabetic db/db Mice.

Authors - Xiao Meng Zhang
humanWB

Cell Death Dis

Mitochondrial dysfunction induces radioresistance in colorectal cancer by activating [Ca2+]m-PDP1-PDH-histone acetylation retrograde signaling.

Authors - Yingying Shi
mouseWB

ACS Chem Neurosci

FDCA Attenuates Neuroinflammation and Brain Injury after Cerebral Ischemic Stroke

Authors - Xin Guan
mouseWB

J Physiol

Monocarboxylate transporter 4 deficiency enhances high-intensity interval training-induced metabolic adaptations in skeletal muscle

Authors - Yuki Tamura