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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PDP1 Recombinant antibody (84612-3-RR)

    WB analysis using 84612-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84612-3-RR (PDP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84612-3-RR

    Affinity and Kinetic Characterization of 84612-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84612-3-RR against Human PDP1 were performed. The affinity constant is below 1 pM.

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PDP1 Recombinant antibody

PDP1 Recombinant Antibody for WB, ELISA
Cat No. 84612-3-RR
Clone No.242090E7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

PPM2C, PDPC 1, PDP 1, EC:3.1.3.43, 242090E7

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMDA-MB-231 cells, PC-3 cells, U-87 MG cells, mouse skeletal muscle tissue, mouse heart tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84612-3-RR targets PDP1 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PDP1 fusion protein Ag15453 相同性解析による交差性が予測される生物種
Full Name protein phosphatase 2C, magnesium-dependent, catalytic subunit
Calculated molecular weight 537 aa, 61 kDa
Observed molecular weight 55-61 kDa
GenBank accession numberBC047619
Gene Symbol PDP1
Gene ID (NCBI) 54704
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9P0J1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Pyruvate dehydrogenase phosphatase 1 (PDP1),also known as PPM2C, is a key hormone-regulated metabolic enzyme that dephosphorylates and activates pyruvate dehydrogenase (PDH), thereby stimulating the conversion of pyruvate into acetyl-CoA (PMID: 36453802). PDP1 expression is regulated by FLT3-ITD in a context-dependent manner, and that it is centrally involved in the adaptation of AML glucose metabolism to the requirements of proliferation, survival and the therapeutic response to FLT3 inhibition (PMID: 37935978).

Protocols

Product Specific Protocols
WB protocol for PDP1 antibody 84612-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols