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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PPP1CA Polyclonal antibody

PPP1CA Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28617-1-AP

Synonyms

PP 1A, PPP1A, PPP1CA

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PPP1CA Polyclonal antibody (28617-1-AP)

    WB analysis using 28617-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28617-1-AP (PPP1CA antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PPP1CA Polyclonal antibody (28617-1-AP)

    IHC staining of human breast cancer using 28617-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28617-1-AP (PPP1CA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PPP1CA Polyclonal antibody (28617-1-AP)

    IHC staining of human breast cancer using 28617-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28617-1-AP (PPP1CA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHeLa cells, LNCaP cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28617-1-AP targets PPP1CA in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PPP1CA fusion protein Ag28451 相同性解析による交差性が予測される生物種
Full Name protein phosphatase 1, catalytic subunit, alpha isoform
Calculated molecular weight 330 aa, 38 kDa
Observed molecular weight37 kDa
GenBank accession numberBC001888
Gene symbol PPP1CA
Gene ID (NCBI) 5499
RRIDAB_2918182
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PPP1CA antibody 28617-1-APDownload protocol
IHC protocol for PPP1CA antibody 28617-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Cancer

Phase separation of phospho-HDAC6 drives aberrant chromatin architecture in triple-negative breast cancer

Authors - Bing Lu
  • KD Validated
View Article