Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, fetal human brain tissue, pig brain tissue, zebrafish tissue, T-47D cells, Jurkat cells, HEK-293 cells, rabbit brain tissue, rat brain tissue, mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 8 publications below |
| IP | See 1 publications below |
Product Information
67070-1-Ig targets PPP1CA in WB, IP, ELISA applications and shows reactivity with Human, mouse, rat, pig, zebrafish, rabbit samples.
| Tested Reactivity | Human, mouse, rat, pig, zebrafish, rabbit |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | PPP1CA fusion protein Ag28451 相同性解析による交差性が予測される生物種 |
| Full Name | protein phosphatase 1, catalytic subunit, alpha isoform |
| Calculated molecular weight | 330 aa, 38 kDa |
| Observed molecular weight | 37 kDa |
| GenBank accession number | BC001888 |
| Gene Symbol | PPP1CA |
| Gene ID (NCBI) | 5499 |
| RRID | AB_2882379 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P62136 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Protein phosphatase associates with over 200 regulatory proteins to form highly specific holoenzymes which dephosphorylate hundreds of biological targets. Type 1 protein phosphatase (PP1), a serine/threonine phosphatase, is essential for cell division and participates in the regulation of glycogen metabolism, muscle contractility, and protein synthesis. Four isoforms of PP1 have been characterized: PP1α, PP1δ, PP1γ1 and PP1γ2 (PMID: 9835651). It has been illustrated that PP1 dephosphorylates Rb and cdc25 during mitosis (PMID: 8384581, PMID: 1392080). A cell cycle-dependent phosphorylation at Thr320 of PP1α by cdc2 kinase inhibits PP1α activity (PMID: 9122166).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PPP1CA antibody 67070-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Inhibited peroxidase activity of peroxiredoxin 1 by palmitic acid exacerbates nonalcoholic steatohepatitis in male mice | ||
Cell Death Differ Lysine methylation of PPP1CA by the methyltransferase SUV39H2 disrupts TFEB-dependent autophagy and promotes intervertebral disc degeneration
| ||
J Neurosci CREB inactivation by HDAC1/PP1γ contributes to dopaminergic neurodegeneration in Parkinson's disease. | ||
Neurotoxicology Nuclear accumulation of histone deacetylase 4 (HDAC4) by PP1-mediated dephosphorylation exerts neurotoxicity in Pb-exposed neural cells. | ||
Exp Ther Med Small interfering RNA-mediated knockdown of KRT80 suppresses colorectal cancer proliferation. | ||
Zool Res EPAS1 prevents telomeric damage-induced senescence by enhancing transcription of TRF1, TRF2, and RAD50
|