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PPP1CA Monoclonal antibody
PPP1CA Monoclonal Antibody for WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, mouse, rat, pig, zebrafish, rabbit and More (1)
Applications
WB, IP, ELISA
Conjugate
Unconjugated
CloneNo.
1C10A2
Cat no : 67070-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, fetal human brain tissue, pig brain tissue, zebrafish tissue, T-47D cells, Jurkat cells, HEK-293 cells, rabbit brain tissue, rat brain tissue, mouse brain tissue |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 2 publications below |
WB | See 7 publications below |
IP | See 1 publications below |
Product Information
67070-1-Ig targets PPP1CA in WB, IP, ELISA applications and shows reactivity with Human, mouse, rat, pig, zebrafish, rabbit samples.
Tested Reactivity | Human, mouse, rat, pig, zebrafish, rabbit |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | PPP1CA fusion protein Ag28451 相同性解析による交差性が予測される生物種 |
Full Name | protein phosphatase 1, catalytic subunit, alpha isoform |
Calculated molecular weight | 330 aa, 38 kDa |
Observed molecular weight | 37 kDa |
GenBank accession number | BC001888 |
Gene symbol | PPP1CA |
Gene ID (NCBI) | 5499 |
RRID | AB_2882379 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Protein phosphatase associates with over 200 regulatory proteins to form highly specific holoenzymes which dephosphorylate hundreds of biological targets. Type 1 protein phosphatase (PP1), a serine/threonine phosphatase, is essential for cell division and participates in the regulation of glycogen metabolism, muscle contractility, and protein synthesis. Four isoforms of PP1 have been characterized: PP1α, PP1δ, PP1γ1 and PP1γ2 (PMID: 9835651). It has been illustrated that PP1 dephosphorylates Rb and cdc25 during mitosis (PMID: 8384581, PMID: 1392080). A cell cycle-dependent phosphorylation at Thr320 of PP1α by cdc2 kinase inhibits PP1α activity (PMID: 9122166).
Protocols
Product Specific Protocols | |
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WB protocol for PPP1CA antibody 67070-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell Death Differ Lysine methylation of PPP1CA by the methyltransferase SUV39H2 disrupts TFEB-dependent autophagy and promotes intervertebral disc degeneration
| ||
J Neurosci CREB inactivation by HDAC1/PP1γ contributes to dopaminergic neurodegeneration in Parkinson's disease. | ||
Neurotoxicology Nuclear accumulation of histone deacetylase 4 (HDAC4) by PP1-mediated dephosphorylation exerts neurotoxicity in Pb-exposed neural cells. | ||
Exp Ther Med Small interfering RNA-mediated knockdown of KRT80 suppresses colorectal cancer proliferation. | ||
Zool Res EPAS1 prevents telomeric damage-induced senescence by enhancing transcription of TRF1, TRF2, and RAD50
| ||
Acta Pharm Sin B Predicting and overcoming resistance to CDK9 inhibitors for cancer therapy |