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  • KD/KO Validated

PPP1CA Monoclonal antibody

PPP1CA Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig, zebrafish, rabbit and More (1)

Applications

WB, IP, ELISA

Conjugate

Unconjugated

CloneNo.

1C10A2

Cat no : 67070-1-Ig

Synonyms

PP 1A, PPP1A, PPP1CA


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Tested Applications

Positive WB detected inHeLa cells, fetal human brain tissue, pig brain tissue, zebrafish tissue, T-47D cells, Jurkat cells, HEK-293 cells, rabbit brain tissue, rat brain tissue, mouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67070-1-Ig targets PPP1CA in WB, IP, ELISA applications and shows reactivity with Human, mouse, rat, pig, zebrafish, rabbit samples.

Tested Reactivity Human, mouse, rat, pig, zebrafish, rabbit
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PPP1CA fusion protein Ag28451 相同性解析による交差性が予測される生物種
Full Name protein phosphatase 1, catalytic subunit, alpha isoform
Calculated molecular weight 330 aa, 38 kDa
Observed molecular weight 37 kDa
GenBank accession numberBC001888
Gene symbol PPP1CA
Gene ID (NCBI) 5499
RRIDAB_2882379
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Protein phosphatase associates with over 200 regulatory proteins to form highly specific holoenzymes which dephosphorylate hundreds of biological targets. Type 1 protein phosphatase (PP1), a serine/threonine phosphatase, is essential for cell division and participates in the regulation of glycogen metabolism, muscle contractility, and protein synthesis. Four isoforms of PP1 have been characterized: PP1α, PP1δ, PP1γ1 and PP1γ2 (PMID: 9835651). It has been illustrated that PP1 dephosphorylates Rb and cdc25 during mitosis (PMID: 8384581, PMID: 1392080). A cell cycle-dependent phosphorylation at Thr320 of PP1α by cdc2 kinase inhibits PP1α activity (PMID: 9122166).

Protocols

Product Specific Protocols
WB protocol for PPP1CA antibody 67070-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IP

Cell Death Differ

Lysine methylation of PPP1CA by the methyltransferase SUV39H2 disrupts TFEB-dependent autophagy and promotes intervertebral disc degeneration

Authors - Huaizhen Liang
  • KD Validated
mouseWB

J Neurosci

CREB inactivation by HDAC1/PP1γ contributes to dopaminergic neurodegeneration in Parkinson's disease.

Authors - Xiaoyi Xu 许潇依
ratWB

Neurotoxicology

Nuclear accumulation of histone deacetylase 4 (HDAC4) by PP1-mediated dephosphorylation exerts neurotoxicity in Pb-exposed neural cells.

Authors - Xiaozhen Gu
humanWB

Exp Ther Med

Small interfering RNA-mediated knockdown of KRT80 suppresses colorectal cancer proliferation.

Authors - Jiatian Lin
human,mouseWB

Zool Res

EPAS1 prevents telomeric damage-induced senescence by enhancing transcription of TRF1, TRF2, and RAD50

Authors - Kai-Qin Li
  • KD Validated
humanWB

Acta Pharm Sin B

Predicting and overcoming resistance to CDK9 inhibitors for cancer therapy

Authors - Chen Hu