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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PPP1CC Monoclonal antibody (68122-1-Ig)

    WB analysis using 68122-1-Ig (same clone as 68122-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68122-1-Ig (PPP1CC antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68122-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of C2C12 cells using PPP1CC Monoclonal antibody (68122-1-Ig)

    IF Staining of C2C12 using 68122-1-Ig (same clone as 68122-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using PPP1CC antibody (68122-1-Ig, Clone: 2G11C3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68122-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PPP1CC Monoclonal antibody (68122-1-Ig)

    IF Staining of HeLa using 68122-1-Ig (same clone as 68122-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PPP1CC antibody (68122-1-Ig, Clone: 2G11C3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68122-1-PBS in a different storage buffer formulation.

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PPP1CC Monoclonal antibody, PBS Only

PPP1CC Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 68122-1-PBS
Clone No.2G11C3

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

PP 1G, PPP1CC, PPP1G

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68122-1-PBS targets PPP1CC in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PPP1CC fusion protein Ag32348 相同性解析による交差性が予測される生物種
Full Name protein phosphatase 1, catalytic subunit, gamma isoform
Calculated molecular weight 35 kDa
Observed molecular weight35 kDa
GenBank accession numberBC014073
Gene Symbol PPP1CC
Gene ID (NCBI) 5501
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP36873
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

PPP1CC, also named as PP-1G, belongs to the PPP phosphatase family and PP-1 subfamily. Protein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. It is involved in regulation of ionic conductances and long-term synaptic plasticity. PPP1CC may play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca2+/calmodulin dependent protein kinase II.