PPP1R10 Polyclonal antibody
PPP1R10 Polyclonal Antibody for WB, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 24450-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HepG2 cells |
Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:200-1:1000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
The immunogen of 24450-1-AP is PPP1R10 Fusion Protein expressed in E. coli.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | PPP1R10 fusion protein Ag20091 相同性解析による交差性が予測される生物種 |
Full Name | protein phosphatase 1, regulatory (inhibitor) subunit 10 |
Calculated molecular weight | 940 aa, 99 kDa |
Observed molecular weight | 99 kDa |
GenBank accession number | BC150310 |
Gene symbol | PPP1R10 |
Gene ID (NCBI) | 5514 |
RRID | AB_2879554 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
Product Specific Protocols | |
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WB protocol for PPP1R10 antibody 24450-1-AP | Download protocol |
IF protocol for PPP1R10 antibody 24450-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nat Cell Biol A regulated PNUTS mRNA to lncRNA splice switch mediates EMT and tumour progression. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Roy (Verified Customer) (06-15-2024) | Perfect to detect PNUTS by WB (1/1000, Overnight incubation at 4°C) and by IF (1/250) in HeLa cells
|
FH Fabienne (Verified Customer) (06-11-2024) | Works well in western blot
|