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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using PPP1R10 Polyclonal antibody (24450-1-AP)

    WB analysis of HepG2 using 24450-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 24450-1-AP (PPP1R10 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PPP1R10 Polyclonal antibody (24450-1-AP)

    IF Staining of HepG2 using 24450-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 24450-1-AP (PPP1R10 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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PPP1R10 Polyclonal antibody

PPP1R10 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 24450-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

CAT53, FB19, p99, PNUTS, PP1 binding protein of 114 kDa, PP1R10, PPP1R10, Protein FB19

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24450-1-AP targets PPP1R10 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PPP1R10 fusion protein Ag20091 相同性解析による交差性が予測される生物種
Full Name protein phosphatase 1, regulatory (inhibitor) subunit 10
Calculated molecular weight 940 aa, 99 kDa
Observed molecular weight 99 kDa
GenBank accession numberBC150310
Gene Symbol PPP1R10
Gene ID (NCBI) 5514
RRIDAB_2879554
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96QC0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PPP1R10 antibody 24450-1-APDownload protocol
IF protocol for PPP1R10 antibody 24450-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Nat Cell Biol

A regulated PNUTS mRNA to lncRNA splice switch mediates EMT and tumour progression.

Authors - Simon Grelet
View Article