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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PPP1R13B Polyclonal antibody (29804-1-AP)

    WB analysis using 29804-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29804-1-AP (PPP1R13B antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using PPP1R13B Polyclonal antibody (29804-1-AP)

    IHC staining of human lung cancer using 29804-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 29804-1-AP (PPP1R13B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using PPP1R13B Polyclonal antibody (29804-1-AP)

    IHC staining of mouse testis using 29804-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29804-1-AP (PPP1R13B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using PPP1R13B Polyclonal antibody (29804-1-AP)

    IHC staining of human lung cancer using 29804-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 29804-1-AP (PPP1R13B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using PPP1R13B Polyclonal antibody (29804-1-AP)

    IHC staining of mouse testis using 29804-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29804-1-AP (PPP1R13B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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PPP1R13B Polyclonal antibody

PPP1R13B Polyclonal Antibody for WB, IHC, ELISA
Cat No. 29804-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse and More (1)

Applications

WB, IHC, ELISA

Synonyms

ASPP1, KIAA0771, p53BP2 like, p85, PPP1R13B

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, MCF-7 cells
Positive IHC detected inhuman lung cancer tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

29804-1-AP targets PPP1R13B in WB, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Cited Reactivity sheep
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PPP1R13B fusion protein Ag30788 相同性解析による交差性が予測される生物種
Full Name protein phosphatase 1, regulatory (inhibitor) subunit 13B
Calculated molecular weight 1090 aa, 120 kDa
Observed molecular weight120 kDa
GenBank accession numberBC136527
Gene Symbol PPP1R13B
Gene ID (NCBI) 23368
RRIDAB_2923610
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96KQ4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

PPP1R13B(Protein phosphatase 1 regulatory subunit 13B), also named ASPP1 and KIAA0771, is a member of the apoptosis-stimulating proteins of the p53 family (ASPPs)(PMID: 30908929). PPP1R13B contains four ankyrin repeats and an SH3 domain involved in protein-protein interactions. PPP1R13 promotes DNA binding and transactivation of p53-family proteins on the promoters of proapoptotic genes. Expression of this gene is regulated by the E2F transcription factor.

Protocols

Product Specific Protocols
WB protocol for PPP1R13B antibody 29804-1-APDownload protocol
IHC protocol for PPP1R13B antibody 29804-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
sheepWB

Int J Biol Macromol

Estrogen-mediated oar-miR-485-5p targets PPP1R13B to regulate myoblast proliferation in sheep

Authors - Siqi Liu
View Article