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KD/KO Validated

iASPP Polyclonal antibody

Name: iASPP Polyclonal antibody
Brand: Proteintech
SKU: 18590-1-AP

iASPP Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 18590-1-AP

Synonyms

PPP1R13L, PPP1R13B-like protein, PPP1R13BL, PPP1R13B like protein, NKIP1

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis using 18590-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 18590-1-AP (iASPP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 18590-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 18590-1-AP (iASPP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 18590-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 18590-1-AP (iASPP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of Apoptosised HeLa using 18590-1-AP
Apoptosised HeLa cells were subjected to SDS PAGE followed by western blot with 18590-1-AP (iASPP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IP experiment of PC-3 using 18590-1-AP
IP result of anti-iASPP (IP:18590-1-AP, 4ug; Detection:18590-1-AP 1:1000) with PC-3 cells lysate 1040ug.
IHC staining of human breast cancer using 18590-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18590-1-AP (iASPP antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 18590-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18590-1-AP (iASPP antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human cervical squamous cancer using 18590-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical squamous cancer tissue slide using 18590-1-AP (iASPP antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of MCF-7 using 18590-1-AP
Immunofluorescent analysis of MCF-7 cells, using PPP1R13L antibody 18590-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).Blue pseudocolor = DAPI (fluorescent DNA dye).

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Cat No. 18590-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	NIH/3T3 cells, PC-3 cells, MCF-7 cells, Apoptosised HeLa cells, C6 cells
Positive IP detected in	PC-3 cells
Positive IHC detected in	human breast cancer tissue, human cervical squamous cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	MCF-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:400-1:1600
Immunofluorescence (IF)/ICC	IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 5 publications below
IF	See 2 publications below
IP	See 2 publications below

Product Information

18590-1-AP targets iASPP in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	iASPP fusion protein Ag13273 相同性解析による交差性が予測される生物種
Full Name	protein phosphatase 1, regulatory (inhibitor) subunit 13 like
Calculated molecular weight	89 kDa
Observed molecular weight	110 kDa
GenBank accession number	BC064913
Gene symbol	PPP1R13L
Gene ID (NCBI)	10848
RRID	AB_2168573
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Inhibitor of apoptosis-stimulating protein of p53 (iASPP), encoded by PPP1R13L gene, is often overexpressed in human cancers. The ASPP family includes three members, namely ASPP1, ASPP2, and iASPP, which are specific regulators of p53-, p63-, and p73-mediated apoptosis. ASPP1 and ASPP2 enhance the apoptotic function of p53, whereas iASPP specifically inhibits p53- mediated apoptosis. Overexpression of iASPP is associated with resistance to cisplatin-induced apoptosis and rediation therapy. iASPP plays a pivotal role in regulating cancer cell proliferation and tumor progression. This antibody could both recognize unphosphorylated and phosphorylated iASPP.

Protocols

Product Specific Protocols
WB protocol for iASPP antibody 18590-1-AP	Download protocol
IHC protocol for iASPP antibody 18590-1-AP	Download protocol
IF protocol for iASPP antibody 18590-1-AP	Download protocol
IP protocol for iASPP antibody 18590-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IP	J Cell Biol iASPP contributes to cell cortex rigidity, mitotic cell rounding, and spindle positioning. Authors - Aurélie Mangon KD Validated View Article
human	WB,IP,IF	Cell Death Dis iASPP-PP1 complex is required for cytokinetic abscission by controlling CEP55 dephosphorylation. Authors - Kun Gao KD Validated View Article
mouse	WB	J Biochem Mol Toxicol Upregulation of iASPP ameliorates hypoxia/reoxygenation-induced apoptosis and oxidative stress in cardiomyocytes by upregulating Nrf2 signaling. Authors - Baobao Bai KD Validated View Article
mouse	WB,IF	Metab Brain Dis Inhibitor of apoptosis stimulating protein of p53 protects against MPP+-induced neurotoxicity of dopaminergic neurons Authors - Lei Chen View Article
mouse	WB	Acta Biochim Biophys Sin (Shanghai) iASPP protects the heart from ischemia injury by inhibiting p53 expression and cardiomyocyte apoptosis. Authors - Timur Yagudin View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

iASPP Polyclonal antibody

iASPP Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis using 18590-1-AP

WB analysis of PC-3 using 18590-1-AP

WB analysis of MCF-7 using 18590-1-AP

WB analysis of Apoptosised HeLa using 18590-1-AP

IP experiment of PC-3 using 18590-1-AP

IHC staining of human breast cancer using 18590-1-AP

IHC staining of human breast cancer using 18590-1-AP

IHC staining of human cervical squamous cancer using 18590-1-AP

IF Staining of MCF-7 using 18590-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

iASPP contributes to cell cortex rigidity, mitotic cell rounding, and spindle positioning.

iASPP-PP1 complex is required for cytokinetic abscission by controlling CEP55 dephosphorylation.

Upregulation of iASPP ameliorates hypoxia/reoxygenation-induced apoptosis and oxidative stress in cardiomyocytes by upregulating Nrf2 signaling.

Inhibitor of apoptosis stimulating protein of p53 protects against MPP+-induced neurotoxicity of dopaminergic neurons

iASPP protects the heart from ischemia injury by inhibiting p53 expression and cardiomyocyte apoptosis.