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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CPI17 Polyclonal antibody

CPI17 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 11658-1-AP

Synonyms

PPP1R14A, Protein kinase C-potentiated inhibitor protein of 17 kDa, PPP1INL, CPI-17, CPI 17

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using CPI17 Polyclonal antibody (11658-1-AP)

    WB analysis of MCF-7 using 11658-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 11658-1-AP (CPI17 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using CPI17 Polyclonal antibody (11658-1-AP)

    WB analysis of HeLa using 11658-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 11658-1-AP (CPI17 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using CPI17 Polyclonal antibody (11658-1-AP)

    WB analysis of Jurkat using 11658-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 11658-1-AP (CPI17 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using CPI17 Polyclonal antibody (11658-1-AP)

    WB analysis of PC-3 using 11658-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 11658-1-AP (CPI17 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human colon tissue using CPI17 Polyclonal antibody (11658-1-AP)

    WB analysis of human colon using 11658-1-AP

    human colon tissue were subjected to SDS PAGE followed by western blot with 11658-1-AP (CPI17 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using CPI17 Polyclonal antibody (11658-1-AP)

    WB analysis of HepG2 using 11658-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 11658-1-AP (CPI17 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using CPI17 Polyclonal antibody (11658-1-AP)

    WB analysis of HepG2 using 11658-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 11658-1-AP (CPI17 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using CPI17 Polyclonal antibody (11658-1-AP)

    IHC staining of human stomach cancer using 11658-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11658-1-AP (CPI17 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using CPI17 Polyclonal antibody (11658-1-AP)

    IF Staining of A549 using 11658-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using CPI17 antibody (11658-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Tested Applications

Positive WB detected inMCF-7 cells, HeLa cells, HepG2 cells, human colon tissue, Jurkat cells, PC-3 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11658-1-AP targets CPI17 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CPI17 fusion protein Ag2237 相同性解析による交差性が予測される生物種
Full Name protein phosphatase 1, regulatory (inhibitor) subunit 14A
Calculated molecular weight 147 aa, 17 kDa
Observed molecular weight 17 kDa
GenBank accession numberBC021089
Gene symbol CPI17
Gene ID (NCBI) 94274
RRIDAB_2252794
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PPP1R14A, also named as CPI17, belongs to the protein phosphatase 1 (PP1) inhibitor family. This protein is an inhibitor of smooth muscle myosin phosphatase, and has higher inhibitory activity when phosphorylated. Inhibition of myosin phosphatase leads to increased myosin phosphorylation and enhanced smooth muscle contraction. CPI17 has two isoforms:CPI-17alpha (17 kDa) and CPI-17beta (13 kDa).

Protocols

Product Specific Protocols
WB protocol for CPI17 antibody 11658-1-APDownload protocol
IHC protocol for CPI17 antibody 11658-1-APDownload protocol
IF protocol for CPI17 antibody 11658-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols