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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PPP1R14B Polyclonal antibody (18476-1-AP)

    WB analysis using 18476-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18476-1-AP (PPP1R14B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using PPP1R14B Polyclonal antibody (18476-1-AP)

    WB analysis of MCF-7 using 18476-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 18476-1-AP (PPP1R14B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using PPP1R14B Polyclonal antibody (18476-1-AP)

    WB analysis of mouse liver using 18476-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 18476-1-AP (PPP1R14B antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using PPP1R14B Polyclonal antibody (18476-1-AP)

    WB analysis of human heart using 18476-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 18476-1-AP (PPP1R14B antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using PPP1R14B Polyclonal antibody (18476-1-AP)

    WB analysis of mouse skeletal muscle using 18476-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 18476-1-AP (PPP1R14B antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PPP1R14B Polyclonal antibody (18476-1-AP)

    IF Staining of HeLa using 18476-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PPP1R14B antibody (18476-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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PPP1R14B Polyclonal antibody

PPP1R14B Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 18476-1-AP
Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

SOM172, PNG, PLCB3N, Phospholipase C-beta-3 neighbouring gene protein, PHI 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMCF-7 cells, mouse liver tissue, human heart tissue, mouse skeletal muscle tissue, U2OS cells, A549 cells, PC-3 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18476-1-AP targets PPP1R14B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PPP1R14B fusion protein Ag13373 相同性解析による交差性が予測される生物種
Full Name protein phosphatase 1, regulatory (inhibitor) subunit 14B
Calculated molecular weight 147 aa, 16 kDa
Observed molecular weight 23 kDa
GenBank accession numberBC014522
Gene Symbol PPP1R14B
Gene ID (NCBI) 26472
RRIDAB_10598019
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96C90
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PPP1R14B antibody 18476-1-APDownload protocol
IF protocol for PPP1R14B antibody 18476-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse

Immunity

Dysregulated Expression of the Nuclear Exosome Targeting Complex Component Rbm7 in Nonhematopoietic Cells Licenses the Development of Fibrosis.

Authors - Kiyoharu Fukushima
View Article
humanWB,IHC

J Cell Mol Med

PPP1R14B is a diagnostic prognostic marker in patients with uterine corpus endometrial carcinoma

Authors - Kang He
View Article
humanWB

Biochim Biophys Acta Mol Basis Dis

Lysine lactylation-based insight to understanding the characterization of cervical cancer

Authors - Chaoran He
View Article