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PPP1R15B Polyclonal antibody
PPP1R15B Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 14634-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | A2780 cells, MCF-7 cells, HEK-293 cells, SGC-7901 cells, MDA-MB-231 cells, SKOV-3 cells |
| Positive IP detected in | MCF-7 cells |
| Positive IHC detected in | mouse brain tissue, mouse cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | SH-SY5Y cells, HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 22 publications below |
| IF | See 3 publications below |
| IP | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
14634-1-AP targets PPP1R15B in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PPP1R15B fusion protein Ag6213 相同性解析による交差性が予測される生物種 |
| Full Name | protein phosphatase 1, regulatory (inhibitor) subunit 15B |
| Calculated molecular weight | 79 kDa |
| Observed molecular weight | 100-110 kDa |
| GenBank accession number | BC065280 |
| Gene symbol | PPP1R15B |
| Gene ID (NCBI) | 84919 |
| RRID | AB_2300036 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Protein phosphatase 1 regulatory subunit 15B(PPP1R15B) is a member of PPP1R15 family, it forms a complex with protein phosphatase1(PP1) and NCK1/2, maintaining low levels of EIF2S1 phosphorylation in unstressed cells, thus is also named constitutive repressor of eIF2alpha phosphorylation (CReP). PPP1R15B is assoicated in endoplasmic reticulum (ER) stress response resulting to a role in translation regulation. Knock-down of PPP1R15B strongly protected mammalian cells against oxidative stress, peroxynitrite stress, and more modestly against accumulation of malfolded proteins in the ER. Recently, PPP1R15B was found to associate with cell membranes and regulate membrane traffic in a PP1c-independent manner, suggesting a novel link between translation and traffic. MW of PPP1R15B is 80 kDa. Observed MW of PPP1R15B is from 100-110 kDa maybe due to phosphorylation (PMID: 28492545).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PPP1R15B antibody 14634-1-AP | Download protocol |
| IHC protocol for PPP1R15B antibody 14634-1-AP | Download protocol |
| IF protocol for PPP1R15B antibody 14634-1-AP | Download protocol |
| IP protocol for PPP1R15B antibody 14634-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Neuron Excitatory neuron-specific suppression of the integrated stress response contributes to autism-related phenotypes in fragile X syndrome | ||
Nat Commun Integration of Hippo signalling and the unfolded protein response to restrain liver overgrowth and tumorigenesis. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Tatyana (Verified Customer) (03-11-2023) | Detects a major band ~100 kDa and shows upregulation in stressed cells (MG132 treatment). WB: 4% milk in TBST and semi-dry transfer; detects a double band at ~55kDa. Also was tested in IHC in mouse CNS tissue and in ICC in human cells; the signal appears specific in both cases (not shown).
 |
FH Will (Verified Customer) (10-24-2019) | good antibody, recommended by a colleague, would order again
|
