• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using PPP1R3A Polyclonal antibody (22495-1-AP)

    WB analysis using 22495-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22495-1-AP (PPP1R3A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of THP-1 cells using PPP1R3A Polyclonal antibody (22495-1-AP)

    IF Staining of THP-1 using 22495-1-AP

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using PPP1R3A antibody (22495-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

PPP1R3A Polyclonal antibody

PPP1R3A Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 22495-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

RG1, Protein phosphatase type-1 glycogen targeting subunit, Protein phosphatase 1 glycogen-associated regulatory subunit, PPP1R3, PP1G

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, THP-1 cells
Positive IF/ICC detected inTHP-1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22495-1-AP targets PPP1R3A in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PPP1R3A fusion protein Ag18013 相同性解析による交差性が予測される生物種
Full Name protein phosphatase 1, regulatory (inhibitor) subunit 3A
Calculated molecular weight 1122 aa, 126 kDa
Observed molecular weight110-130 kDa
GenBank accession numberBC126453
Gene Symbol PPP1R3A
Gene ID (NCBI) 5506
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ16821
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PPP1R3A (Protein Phosphatase 1 Regulatory Subunit 3A), also known as Protein Targeting to Glycogen (PTG), is a regulatory subunit of protein phosphatase 1 (PP1), a major serine/threonine phosphatase involved in metabolic regulation. It plays a crucial role in glycogen metabolism by targeting PP1 to glycogen particles, facilitating glycogen synthesis and breakdown. PPP1R3A is involved in muscle glycogen regulation (PMID: 24264575).

Protocols

Product Specific Protocols
WB protocol for PPP1R3A antibody 22495-1-APDownload protocol
IF protocol for PPP1R3A antibody 22495-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols