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  • Featured Product
  • KD/KO Validated

PPP1R3B Polyclonal antibody

PPP1R3B Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14190-1-AP

Synonyms

PTG, PPP1R4, PP1 subunit R4, Hepatic glycogen-targeting protein phosphatase 1 regulatory subunit GL

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using PPP1R3B Polyclonal antibody (14190-1-AP)

    WB analysis of mouse liver using 14190-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 14190-1-AP (PPP1R3B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using PPP1R3B Polyclonal antibody (14190-1-AP)

    IHC staining of human stomach cancer using 14190-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer slide using 14190-1-AP (PPP1R3B antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using PPP1R3B Polyclonal antibody (14190-1-AP)

    IHC staining of human stomach cancer using 14190-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer slide using 14190-1-AP (PPP1R3B antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse liver tissue
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14190-1-AP targets PPP1R3B in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PPP1R3B fusion protein Ag5521 相同性解析による交差性が予測される生物種
Full Name protein phosphatase 1, regulatory (inhibitor) subunit 3B
Calculated molecular weight 33 kDa
Observed molecular weight 33 kDa
GenBank accession numberBC043388
Gene symbol PPP1R3B
Gene ID (NCBI) 79660
RRIDAB_2878026
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PPP1R3B antibody 14190-1-APDownload protocol
IHC protocol for PPP1R3B antibody 14190-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Rep

PMID: 31553910

Authors - Qiqi Li
  • KD Validated
View Article