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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PPP1R3C Polyclonal antibody

PPP1R3C Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29424-1-AP

Synonyms

PP1 subunit R5, PPP1R3C, PPP1R5, Protein targeting to glycogen, PTG

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PPP1R3C Polyclonal antibody (29424-1-AP)

    WB analysis using 29424-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29424-1-AP (PPP1R3C antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PPP1R3C Polyclonal antibody (29424-1-AP)

    IHC staining of human colon cancer using 29424-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29424-1-AP (PPP1R3C antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PPP1R3C Polyclonal antibody (29424-1-AP)

    IHC staining of human colon cancer using 29424-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29424-1-AP (PPP1R3C antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHEK-293T cells, mouse cerebellum tissue, rat cerebellum tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29424-1-AP targets PPP1R3C in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PPP1R3C fusion protein Ag30280 相同性解析による交差性が予測される生物種
Full Name protein phosphatase 1, regulatory (inhibitor) subunit 3C
Calculated molecular weight 317 aa, 36 kDa
GenBank accession numberBC012625
Gene symbol PPP1R3C
Gene ID (NCBI) 5507
RRIDAB_2918304
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

• Protein phosphatase 1 regulatory subunit 3C (PPP1R3C) is an enzyme that binds to protein phosphatase-1 (PP1) as a regulator. The central role of PPP1R3C is associated with glycogenesis and the overexpression of this gene increases glycogen storage. The cellular glycogen level is suppressed when PPP1R3C is knocked-down. PPP1R3C induces glycogen accumulation via HIF1 α during hypoxia and it is hypermethylated in melanoma (PMID: 25846879). Overexpression of PPP1R3C increased hepatic gluconeogenesis in vitro and in vivo while knockdown of PPP1R3C showed an opposite result (PMID: 31181215).

Protocols

Product Specific Protocols
WB protocol for PPP1R3C antibody 29424-1-APDownload protocol
IHC protocol for PPP1R3C antibody 29424-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IHC

J Mol Med (Berl)

Prefrontal cortex astrocytes in major depressive disorder: exploring pathogenic mechanisms and potential therapeutic targets

Authors - Yarui Pan
View Article