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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PPP1R3C Polyclonal antibody (29424-1-AP)

    WB analysis using 29424-1-AP (same clone as 29424-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29424-1-AP (PPP1R3C antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29424-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PPP1R3C Polyclonal antibody (29424-1-AP)

    IHC staining of human colon cancer using 29424-1-AP (same clone as 29424-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29424-1-AP (PPP1R3C antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29424-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PPP1R3C Polyclonal antibody (29424-1-AP)

    IHC staining of human colon cancer using 29424-1-AP (same clone as 29424-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29424-1-AP (PPP1R3C antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29424-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

29424-1-PBS targets PPP1R3C in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PPP1R3C fusion protein Ag30280 相同性解析による交差性が予測される生物種
Full Name protein phosphatase 1, regulatory (inhibitor) subunit 3C
Calculated molecular weight 317 aa, 36 kDa
GenBank accession numberBC012625
Gene Symbol PPP1R3C
Gene ID (NCBI) 5507
RRIDAB_2918304
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UQK1
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

• Protein phosphatase 1 regulatory subunit 3C (PPP1R3C) is an enzyme that binds to protein phosphatase-1 (PP1) as a regulator. The central role of PPP1R3C is associated with glycogenesis and the overexpression of this gene increases glycogen storage. The cellular glycogen level is suppressed when PPP1R3C is knocked-down. PPP1R3C induces glycogen accumulation via HIF1 α during hypoxia and it is hypermethylated in melanoma (PMID: 25846879). Overexpression of PPP1R3C increased hepatic gluconeogenesis in vitro and in vivo while knockdown of PPP1R3C showed an opposite result (PMID: 31181215).