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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PPP1R8 Polyclonal antibody

PPP1R8 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16115-1-AP

Synonyms

NIPP1, NIPP 1, ARD-1, ARD1, ARD 1

Validation Data Gallery

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  • Western Blot (WB) analysis of PC-3 cells using PPP1R8 Polyclonal antibody (16115-1-AP)

    WB analysis of PC-3 using 16115-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 16115-1-AP (PPP1R8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using PPP1R8 Polyclonal antibody (16115-1-AP)

    WB analysis of HepG2 using 16115-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16115-1-AP (PPP1R8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using PPP1R8 Polyclonal antibody (16115-1-AP)

    IHC staining of human kidney using 16115-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 16115-1-AP (PPP1R8 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using PPP1R8 Polyclonal antibody (16115-1-AP)

    IHC staining of human kidney using 16115-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 16115-1-AP (PPP1R8 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using PPP1R8 Polyclonal antibody (16115-1-AP)

    IHC staining of human testis using 16115-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 16115-1-AP (PPP1R8 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using PPP1R8 Polyclonal antibody (16115-1-AP)

    IHC staining of human testis using 16115-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 16115-1-AP (PPP1R8 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using PPP1R8 Polyclonal antibody (16115-1-AP)

    IHC staining of human spleen using 16115-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 16115-1-AP (PPP1R8 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using PPP1R8 Polyclonal antibody (16115-1-AP)

    IHC staining of human spleen using 16115-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 16115-1-AP (PPP1R8 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PPP1R8 Polyclonal antibody (16115-1-AP)

    IF Staining of HepG2 using 16115-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 16115-1-AP (PPP1R8 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inPC-3 cells, HepG2 cells
Positive IHC detected inhuman kidney tissue, human spleen tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16115-1-AP targets PPP1R8 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PPP1R8 fusion protein Ag9041 相同性解析による交差性が予測される生物種
Full Name protein phosphatase 1, regulatory (inhibitor) subunit 8
Calculated molecular weight 209 aa, 23 kDa
Observed molecular weight 47 kDa, 28 kDa, 19 kDa
GenBank accession numberBC013360
Gene symbol PPP1R8
Gene ID (NCBI) 5511
RRIDAB_2169304
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PPP1R8, also named as ARD1, NIPP1, is a 351 amino acid protein, which has a basic N- and C-terminal and an acidic central domain. PPP1R8 is ubiquitously expressed, with highest levels in heart and skeletal muscle, followed by brain, placenta, lung, liver and pancreas. PPP1R8 has RNA-binding activity but does not cleave RNA and may target PP-1 to RNA-associated substrates and may also be involved in pre-mRNA splicing. PPP1R8 exists many isoforms and molecular weight of modified isoforms is about 18 kDa, 28 kDa and 47 kDa.

Protocols

Product Specific Protocols
WB protocol for PPP1R8 antibody 16115-1-APDownload protocol
IHC protocol for PPP1R8 antibody 16115-1-APDownload protocol
IF protocol for PPP1R8 antibody 16115-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols