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PPP2CA Polyclonal antibody

Name: PPP2CA Polyclonal antibody
Brand: Proteintech
SKU: 13482-1-AP

PPP2CA Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 13482-1-AP

Synonyms

Replication protein C, PP2CA, PP2Ac, PP2A-alpha, PP2A alpha

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICCFC (Intra)

WB analysis using 13482-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13482-1-AP (PPP2CA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 13482-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 13482-1-AP (PPP2CA antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 13482-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 13482-1-AP (PPP2CA antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293T using 13482-1-AP
HEK-293T cells were subjected to SDS PAGE followed by western blot with 13482-1-AP (PPP2CA antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of human liver using 13482-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 13482-1-AP (PPP2CA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IP experiment of Jurkat using 13482-1-AP
IP result of anti-PPP2CA (IP:13482-1-AP, 4ug; Detection:13482-1-AP 1:500) with Jurkat cells lysate 2400ug.
IHC staining of human liver using 13482-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13482-1-AP (PPP2CA antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human liver using 13482-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13482-1-AP (PPP2CA antibody) at dilution of 1:200 (under 40x lens).
IF Staining of MCF-7 using 13482-1-AP
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PPP2CA antibody (13482-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
FC experiment of MCF-7 using 13482-1-AP
1X10^6 MCF-7 cells were intracellularly stained with 0.2 ug Anti-Human PPP2CA (13482-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Cat No. 13482-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HEK-293T cells, human liver tissue, Jurkat cells, MCF-7 cells
Positive IP detected in	Jurkat cells
Positive IHC detected in	human liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	MCF-7 cells
Positive FC (Intra) detected in	MCF-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 5 publications below
WB	See 32 publications below
IHC	See 1 publications below
IF	See 2 publications below
IP	See 4 publications below
CoIP	See 1 publications below

Product Information

13482-1-AP targets PPP2CA in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PPP2CA fusion protein Ag4294 相同性解析による交差性が予測される生物種
Full Name	protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform
Calculated molecular weight	309 aa, 36 kDa
Observed molecular weight	36 kDa
GenBank accession number	BC031696
Gene symbol	PPP2CA
Gene ID (NCBI)	5515
RRID	AB_2169485
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PPP2CA, also named as RP-C and PP2A-alpha, belongs to the PPP phosphatase family and PP-1 subfamily. PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. PP2A activity was modestly decreased in AML patients harboring C-KIT/WT.PP2A is a target for C-KIT/TKD and implicated the potential efficacy of therapies targeting PP2A in such AML in future.

Protocols

Product Specific Protocols
WB protocol for PPP2CA antibody 13482-1-AP	Download protocol
IHC protocol for PPP2CA antibody 13482-1-AP	Download protocol
IF protocol for PPP2CA antibody 13482-1-AP	Download protocol
IP protocol for PPP2CA antibody 13482-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Science Identification of Integrator-PP2A complex (INTAC), an RNA polymerase II phosphatase. Authors - Hai Zheng View Article
mouse	WB	Protein Cell Senktide blocks aberrant RTN3 interactome to retard memory decline and tau pathology in social isolated Alzheimer's disease mice Authors - He-Zhou Huang View Article
human	WB,IP	Cell Res Nuclear UHRF1 is a gate-keeper of cellular AMPK activity and function. Authors - Xiang Xu KD Validated View Article
human	WB	Acta Pharm Sin B WSB1 regulates c-Myc expression through β-catenin signaling and forms a feedforward circuit. Authors - Xiaomeng Gao View Article
mouse	WB	Cardiovasc Res Role of type 2A phosphatase regulatory subunit B56α in regulating cardiac responses to β-adrenergic stimulation in vivo. Authors - Sarah-Lena Puhl View Article
human	WB	EMBO J The CEP170B-KIF2A complex destabilizes microtubule minus ends to generate polarized microtubule network Authors - Cuirong Guan View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PPP2CA Polyclonal antibody

PPP2CA Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Validation Data Gallery

WB analysis using 13482-1-AP

WB analysis of Jurkat using 13482-1-AP

WB analysis of MCF-7 using 13482-1-AP

WB analysis of HEK-293T using 13482-1-AP

WB analysis of human liver using 13482-1-AP

IP experiment of Jurkat using 13482-1-AP

IHC staining of human liver using 13482-1-AP

IHC staining of human liver using 13482-1-AP

IF Staining of MCF-7 using 13482-1-AP

FC experiment of MCF-7 using 13482-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Identification of Integrator-PP2A complex (INTAC), an RNA polymerase II phosphatase.

Senktide blocks aberrant RTN3 interactome to retard memory decline and tau pathology in social isolated Alzheimer's disease mice

Nuclear UHRF1 is a gate-keeper of cellular AMPK activity and function.

WSB1 regulates c-Myc expression through β-catenin signaling and forms a feedforward circuit.

Role of type 2A phosphatase regulatory subunit B56α in regulating cardiac responses to β-adrenergic stimulation in vivo.

The CEP170B-KIF2A complex destabilizes microtubule minus ends to generate polarized microtubule network