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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PPP2CA Recombinant antibody

PPP2CA Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241112F3

Cat no : 84155-6-RR

Synonyms

PP2CA, PP2Ac, PP2A-alpha, PP2A alpha, EC:3.1.3.16

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using PPP2CA Recombinant antibody (84155-6-RR)

    IF Staining of MCF-7 using 84155-6-RR

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PPP2CA antibody (84155-6-RR, Clone: 241112F3 ) at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of HeLa cells using PPP2CA Recombinant antibody (84155-6-RR)

    FC experiment of HeLa using 84155-6-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug PPP2CA Recombinant antibody (84155-6-RR, Clone:241112F3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 84155-6-RR

    Affinity and Kinetic Characterization of 84155-6-RR

    Biolayer interferometry (BLl) kinetic assays of 84155-6-RR against Human PPP2CA were performed. The affinity constant is 24.4 nM.

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Tested Applications

Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84155-6-RR is PPP2CA Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PPP2CA fusion protein Ag4294 相同性解析による交差性が予測される生物種
Full Name protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform
Calculated molecular weight 309 aa, 36 kDa
GenBank accession numberBC031696
Gene symbol PPP2CA
Gene ID (NCBI) 5515
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PPP2CA, also named as RP-C and PP2A-alpha, belongs to the PPP phosphatase family and PP-1 subfamily. PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. PP2A activity was modestly decreased in AML patients harboring C-KIT/WT.PP2A is a target for C-KIT/TKD and implicated the potential efficacy of therapies targeting PP2A in such AML in future.

Protocols

Product Specific Protocols
IF protocol for PPP2CA antibody 84155-6-RRDownload protocol
FC protocol for PPP2CA antibody 84155-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols