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Species Reactivity

Species Reactivity

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  • Featured Product
  • KD/KO Validated

PPP2CB Polyclonal antibody

PPP2CB Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 12554-2-AP

Synonyms

Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform, PP2CB, PP2A-beta, PP2A beta, EC:3.1.3.16

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse lung tissue using PPP2CB Polyclonal antibody (12554-2-AP)

    WB analysis of mouse lung using 12554-2-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 12554-2-AP (PPP2CB antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of U2OS cells using PPP2CB Polyclonal antibody (12554-2-AP)

    WB analysis of U2OS using 12554-2-AP

    U2OS cells were subjected to SDS PAGE followed by western blot with 12554-2-AP (PPP2CB Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of NIH/3T3 cells using PPP2CB Polyclonal antibody (12554-2-AP)

    IP experiment of NIH/3T3 using 12554-2-AP

    IP result of anti-PPP2CB (IP:12554-2-AP, 4ug; Detection:12554-2-AP 1:300) with NIH/3T3 cells lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using PPP2CB Polyclonal antibody (12554-2-AP)

    IHC staining of human stomach cancer using 12554-2-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12554-2-AP (PPP2CB antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).

  • Immunohistochemistry (IHC) staining of rat kidney tissue using PPP2CB Polyclonal antibody (12554-2-AP)

    IHC staining of rat kidney using 12554-2-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 12554-2-AP (PPP2CB antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse lung tissue, U2OS cells
Positive IP detected inNIH/3T3 cells
Positive IHC detected inrat kidney tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12554-2-AP targets PPP2CB in WB, IHC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PPP2CB fusion protein Ag3233 相同性解析による交差性が予測される生物種
Full Name protein phosphatase 2 (formerly 2A), catalytic subunit, beta isoform
Calculated molecular weight 309 aa, 36 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC012022
Gene symbol PPP2CB
Gene ID (NCBI) 5516
RRIDAB_2169651
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PPP2CB antibody 12554-2-APDownload protocol
IHC protocol for PPP2CB antibody 12554-2-APDownload protocol
IP protocol for PPP2CB antibody 12554-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,CoIP

Cell Mol Life Sci

GADD45α alleviates acetaminophen-induced hepatotoxicity by promoting AMPK activation.

Authors - Chunmin Li
  • KD Validated
View Article
humanWB

Front Cell Dev Biol

BRG1 Activates PR65A Transcription to Regulate NO Bioavailability in Vascular Endothelial Cells.

Authors - Baoyu Chen
View Article
humanWB,CoIP

Cell Signal

METTL14-mediated N6-methyladenosine modification of TCP1 mRNA promotes acute myeloid leukemia progression

Authors - Mengmeng Zhang
View Article