Featured Product
KD/KO Validated

PPP2R1B Polyclonal antibody

Name: PPP2R1B Polyclonal antibody
Brand: Proteintech
SKU: 12621-1-AP

PPP2R1B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 12621-1-AP

Synonyms

Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A beta isoform, PR65B, PP2A subunit A isoform R1-beta, PP2A subunit A isoform R1 beta, PP2A subunit A isoform PR65-beta

Validation Data Gallery

Filter:

AllWBIHCIF/ICC

WB analysis using 12621-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12621-1-AP (PPP2R1B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis using 12621-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12621-1-AP (PPP2R1B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of human kidney using 12621-1-AP
human kidney tissue were subjected to SDS PAGE followed by western blot with 12621-1-AP (PPP2R1B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC staining of human liver using 12621-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12621-1-AP (PPP2R1B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 12621-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12621-1-AP (PPP2R1B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of Caco-2 using 12621-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed Caco-2 cells using PPP2R1B antibody (12621-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

-/ -

Cat No. 12621-1-AP

Size/Concentration

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	A2780 cells, human kidney tissue, mouse testis tissue, rat testis tissue, HeLa cells
Positive IHC detected in	human liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	Caco-2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 3 publications below
IHC	See 1 publications below
CoIP	See 1 publications below

Product Information

12621-1-AP targets PPP2R1B in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PPP2R1B fusion protein Ag3307 相同性解析による交差性が予測される生物種
Full Name	protein phosphatase 2 (formerly 2A), regulatory subunit A, beta isoform
Calculated molecular weight	667 aa, 74 kDa
Observed molecular weight	60-74 kDa
GenBank accession number	BC027596
Gene symbol	PPP2R1B
Gene ID (NCBI)	5519
RRID	AB_2169941
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for PPP2R1B antibody 12621-1-AP	Download protocol
IHC protocol for PPP2R1B antibody 12621-1-AP	Download protocol
IF protocol for PPP2R1B antibody 12621-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Front Cell Dev Biol BRG1 Activates PR65A Transcription to Regulate NO Bioavailability in Vascular Endothelial Cells. Authors - Baoyu Chen View Article
mouse	WB	EMBO Rep CIP2A deficiency promotes depression-like behaviors in mice through inhibition of dendritic arborization Authors - Wen-Ting Hu View Article
human,mouse	WB,IHC,CoIP	Cancer Cell Int PPP2R1B abolishes colorectal cancer liver metastasis and sensitizes Oxaliplatin by inhibiting MAPK/ERK signaling pathway Authors - Wei Liu KD Validated View Article

製品

アプリケーション

cGMPグレード製品

サポート

カスタム対応

プロモーション

企業情報

抗体

免疫アッセイ

サイトカイン・増殖因子

ChromoTek VHH（Nanobody®）試薬

サポートツール・試薬

ゲノミクス試薬

研究分野

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PPP2R1B Polyclonal antibody

PPP2R1B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Validation Data Gallery

WB analysis using 12621-1-AP

WB analysis using 12621-1-AP

WB analysis of human kidney using 12621-1-AP

IHC staining of human liver using 12621-1-AP

IHC staining of human liver using 12621-1-AP

IF Staining of Caco-2 using 12621-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

BRG1 Activates PR65A Transcription to Regulate NO Bioavailability in Vascular Endothelial Cells.

CIP2A deficiency promotes depression-like behaviors in mice through inhibition of dendritic arborization

PPP2R1B abolishes colorectal cancer liver metastasis and sensitizes Oxaliplatin by inhibiting MAPK/ERK signaling pathway