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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PPP2R2A/B/C Monoclonal antibody (67783-1-Ig)

    WB analysis using 67783-1-Ig (same clone as 67783-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67783-1-Ig (PPP2R2A/B/C antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67783-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PPP2R2A/B/C Monoclonal antibody (67783-1-Ig)

    WB analysis using 67783-1-Ig (same clone as 67783-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67783-1-Ig (PPP2R2A/B/C antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67783-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PPP2R2A/B/C Monoclonal antibody (67783-1-Ig)

    IHC staining of mouse brain using 67783-1-Ig (same clone as 67783-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67783-1-Ig (PPP2R2A/B/C antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67783-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PPP2R2A/B/C Monoclonal antibody (67783-1-Ig)

    IHC staining of mouse brain using 67783-1-Ig (same clone as 67783-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67783-1-Ig (PPP2R2A/B/C antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67783-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using PPP2R2A/B/C Monoclonal antibody (67783-1-Ig)

    IHC staining of mouse cerebellum using 67783-1-Ig (same clone as 67783-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67783-1-Ig (PPP2R2A/B/C antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67783-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using PPP2R2A/B/C Monoclonal antibody (67783-1-Ig)

    IHC staining of mouse cerebellum using 67783-1-Ig (same clone as 67783-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67783-1-Ig (PPP2R2A/B/C antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67783-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using PPP2R2A/B/C Monoclonal antibody (67783-1-Ig)

    IF Staining of SH-SY5Y using 67783-1-Ig (same clone as 67783-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using PPP2R2A/B/C antibody (67783-1-Ig, Clone: 2E1D5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67783-1-PBS in a different storage buffer formulation.

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PPP2R2A/B/C Monoclonal antibody, PBS Only

PPP2R2A/B/C Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67783-1-PBS
Clone No.2E1D5

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

PPP2R2B, PP2A subunit B isoform B55-beta, PP2A PR55B, PP2A B55BETA, B55β

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67783-1-PBS targets PPP2R2A/B/C in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PPP2R2A/B/C fusion protein Ag30774 相同性解析による交差性が予測される生物種
Full Name protein phosphatase 2 (formerly 2A), regulatory subunit B, beta isoform
Calculated molecular weight 443 aa, 52 kDa
Observed molecular weight52 kDa
GenBank accession numberBC031790
Gene Symbol PPP2R2B
Gene ID (NCBI) 5521
RRIDAB_2918547
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ00005
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

The PPP2R2B gene encodes a deduced 443 amino acid protein of approximately 52 kDa, which is a brain-specific regulatory subunit B of protein phosphatase 2. PPP2R2B is a Subunit of PP2A, a highly conserved constitutive enzyme(PMID:11719278). PPP2R2B (Bβ) is an important regulator of protein phosphatase 2A (PP2A) activity in the brain. Through differential promoter usage and alternative splicing, two major isoforms Bβ1 and Bβ2 with divergent sub-cellular targeting N termini are produced. Bβ plays an important role in neuronal survival. It has 5 isoforms produced by alternative splicing. This antibody can recognize PPP2R2A and PPP2R2C due to the immunogen of PPP2R2B having high homology with PPP2R2A and PPP2R2C.