Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, SH-SY5Y cells, MCF-7 cells, mouse brain tissue, HepG2 cells, fetal human brain tissue, rat brain tissue, Jurkat cells |
| Positive IP detected in | SH-SY5Y cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
13123-1-AP targets PPP2R2B/A/C/D in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PPP2R2B/A/C/D fusion protein Ag3860 相同性解析による交差性が予測される生物種 |
| Full Name | protein phosphatase 2 (formerly 2A), regulatory subunit B, beta isoform |
| Calculated molecular weight | 443 aa, 52 kDa |
| Observed molecular weight | 52 kDa |
| GenBank accession number | BC031790 |
| Gene Symbol | PPP2R2B |
| Gene ID (NCBI) | 5521 |
| RRID | AB_2169964 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q00005 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The PPP2R2B gene encodes a deduced 443-amino acid protein of approximately 52 kDa,which is a brain-specific regulatory subunit B of protein phosphatase 2. PPP2R2B is a Subunit of PP2A, a highly conserved constitutive enzyme(PMID:11719278). PPP2R2B (Bβ) is an important regulator of protein phosphatase 2A (PP2A) activity in the brain. Through differential promoter usage and alternative splicing, two major isoforms Bβ1 and Bβ2 with divergent sub-cellular targeting N termini are produced. Bβ plays an important role in neuronal survival. It has 5 isoforms produced by alternative splicing. This antibody has cross-reactivity to PPP2R2A, PPP2R2C and PPP2R2D.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PPP2R2B/A/C/D antibody 13123-1-AP | Download protocol |
| IHC protocol for PPP2R2B/A/C/D antibody 13123-1-AP | Download protocol |
| IF protocol for PPP2R2B/A/C/D antibody 13123-1-AP | Download protocol |
| IP protocol for PPP2R2B/A/C/D antibody 13123-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Ther Nucleic Acids miR21 modulates the Hippo signaling pathway via interference with PP2A Bβ to inhibit trophoblast invasion and cause preeclampsia | ||
Cancer Cell Int PPP2R2B downregulation is associated with immune evasion and predicts poor clinical outcomes in triple-negative breast cancer. |