PPP2R2C Polyclonal antibody

Name: PPP2R2C Polyclonal antibody
Brand: Proteintech
SKU: 12747-1-AP

PPP2R2C Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, CoIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 12747-1-AP

Synonyms

B55 GAMMA, IMYPNO, IMYPNO1, PP2A subunit B isoform gamma, PPP2R2C, PR52, PR55G

Validation Data Gallery

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AllWBIHC

WB analysis of HeLa using 12747-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 12747-1-AP (PPP2R2C Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 12747-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 12747-1-AP (PPP2R2C Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 12747-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 12747-1-AP (PPP2R2C antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IHC staining of human breast cancer using 12747-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 12747-1-AP (PPP2R2C antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human breast cancer using 12747-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 12747-1-AP (PPP2R2C antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human testis using 12747-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 12747-1-AP (PPP2R2C antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human testis using 12747-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 12747-1-AP (PPP2R2C antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human brain using 12747-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 12747-1-AP (PPP2R2C antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human brain using 12747-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 12747-1-AP (PPP2R2C antibody) at dilution of 1:50 (under 40x lens).

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Cat No. 12747-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, MCF-7 cells, mouse testis tissue
Positive IHC detected in	human breast cancer tissue, human brain tissue, human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 5 publications below
CoIP	See 1 publications below

Product Information

12747-1-AP targets PPP2R2C in WB, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PPP2R2C fusion protein Ag3470 相同性解析による交差性が予測される生物種
Full Name	protein phosphatase 2 (formerly 2A), regulatory subunit B, gamma isoform
Calculated molecular weight	430 aa, 49 kDa
Observed molecular weight	49 kDa
GenBank accession number	BC032954
Gene symbol	PPP2R2C
Gene ID (NCBI)	5522
RRID	AB_2170099
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for PPP2R2C antibody 12747-1-AP	Download protocol
IHC protocol for PPP2R2C antibody 12747-1-AP	Download protocol
FC protocol for PPP2R2C antibody 12747-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Protein Cell Senktide blocks aberrant RTN3 interactome to retard memory decline and tau pathology in social isolated Alzheimer's disease mice Authors - He-Zhou Huang View Article
human		J Cell Sci The role of specific PP2A complexes in the dephosphorylation of γ-H2AX. Authors - Liping Chen View Article
mouse	WB	J Biochem Involvement of PP2A Methylation in the Adipogenic Differentiation of Bone Marrow Derived Mesenchymal Stem Cell. Authors - Shunta Ikeda View Article
mouse	WB	Biochim Biophys Acta Mol Cell Res Hsc70 phosphorylation patterns and calmodulin regulate AP2 CCV life span for cell adhesion protein transport Authors - G F Sengül View Article
human	WB	Toxicol Appl Pharmacol Chronic arsenic exposure suppresses ATM pathway activation in human keratinocytes Authors - Alexandra N Nail View Article
human	WB,CoIP	Cell Signal METTL14-mediated N6-methyladenosine modification of TCP1 mRNA promotes acute myeloid leukemia progression Authors - Mengmeng Zhang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PPP2R2C Polyclonal antibody

PPP2R2C Polyclonal Antibody for WB, IHC, ELISA

Validation Data Gallery

WB analysis of HeLa using 12747-1-AP

WB analysis of mouse testis using 12747-1-AP

WB analysis of MCF-7 using 12747-1-AP

IHC staining of human breast cancer using 12747-1-AP

IHC staining of human breast cancer using 12747-1-AP

IHC staining of human testis using 12747-1-AP

IHC staining of human testis using 12747-1-AP

IHC staining of human brain using 12747-1-AP

IHC staining of human brain using 12747-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Senktide blocks aberrant RTN3 interactome to retard memory decline and tau pathology in social isolated Alzheimer's disease mice

The role of specific PP2A complexes in the dephosphorylation of γ-H2AX.

Involvement of PP2A Methylation in the Adipogenic Differentiation of Bone Marrow Derived Mesenchymal Stem Cell.

Hsc70 phosphorylation patterns and calmodulin regulate AP2 CCV life span for cell adhesion protein transport

Chronic arsenic exposure suppresses ATM pathway activation in human keratinocytes

METTL14-mediated N6-methyladenosine modification of TCP1 mRNA promotes acute myeloid leukemia progression