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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using PPP2R3C Polyclonal antibody (24877-1-AP)

    WB analysis of mouse brain using 24877-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 24877-1-AP (PPP2R3C Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using PPP2R3C Polyclonal antibody (24877-1-AP)

    WB analysis of rat brain using 24877-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 24877-1-AP (PPP2R3C Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human brain tissue using PPP2R3C Polyclonal antibody (24877-1-AP)

    IHC staining of human brain using 24877-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24877-1-AP (PPP2R3C Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using PPP2R3C Polyclonal antibody (24877-1-AP)

    IHC staining of human brain using 24877-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24877-1-AP (PPP2R3C Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using PPP2R3C Polyclonal antibody (24877-1-AP)

    IHC staining of human stomach cancer using 24877-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 24877-1-AP (PPP2R3C Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using PPP2R3C Polyclonal antibody (24877-1-AP)

    IHC staining of human stomach cancer using 24877-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 24877-1-AP (PPP2R3C Antibody) at dilution of 1:50 (under 40x lens).

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PPP2R3C Polyclonal antibody

PPP2R3C Polyclonal Antibody for WB, IHC, ELISA
Cat No. 24877-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit gamma, Rhabdomyosarcoma antigen MU-RMS-40.6A/6C, G5PR, G4 1, C14orf10

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inhuman brain tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24877-1-AP targets PPP2R3C in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PPP2R3C fusion protein Ag19282 相同性解析による交差性が予測される生物種
Full Name protein phosphatase 2 (formerly 2A), regulatory subunit B'', gamma
Calculated molecular weight 453 aa, 53 kDa
Observed molecular weight 53 kDa
GenBank accession numberBC063438
Gene Symbol PPP2R3C
Gene ID (NCBI) 55012
RRIDAB_2879773
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ969Q6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PPP2R3C(Serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit gamma) is also named as C14orf10, and G5PR. It may regulate MCM3AP phosphorylation through phosphatase recruitment and play a role in the activation-induced cell death of B-cells. It has 2 isoforms produced by alternative splicing with the molecular weight of 53 kDa and 40 kDa.

Protocols

Product Specific Protocols
WB protocol for PPP2R3C antibody 24877-1-APDownload protocol
IHC protocol for PPP2R3C antibody 24877-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols