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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PPP2R5A Polyclonal antibody (29365-1-AP)

    WB analysis using 29365-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29365-1-AP (PPP2R5A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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PPP2R5A Polyclonal antibody

PPP2R5A Polyclonal Antibody for WB, ELISA
Cat No. 29365-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

WB, ELISA

Synonyms

B56A, PP2A B subunit isoform B alpha, PPP2R5A, PR61A, PR61alpha

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, mouse heart tissue, rat heart tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29365-1-AP targets PPP2R5A in WB, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PPP2R5A fusion protein Ag31072 相同性解析による交差性が予測される生物種
Full Name protein phosphatase 2, regulatory subunit B', alpha isoform
Calculated molecular weight 486 aa, 56 kDa
Observed molecular weight 50-56 kDa
GenBank accession numberBC022474
Gene Symbol PPP2R5A
Gene ID (NCBI) 5525
RRIDAB_3086125
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15172
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Protein phosphatase 2, regulatory subunit B (B56), alpha isoform (PPP2R5A) is one of the regulatory subunits of the protein phosphatase 2A (PP2A), which is a major member of protein serine/threonine phosphatases in cells. PPP2R5A can regulate the cellular location, substrate specification and protein phosphatase function of PP2A, through which PPP2R5A plays an important role in many cellular activities. It has been reported that PPP2R5A relates with many diseases including cancers by regulating many crucial signaling pathways involved in P53, Bcl-2, CDK, MAPK, JAK/STAT, c-Myc and β-Catenin.

Protocols

Product Specific Protocols
WB protocol for PPP2R5A antibody 29365-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols